{"product_id":"abcam-ab100546","title":"Abcam, ab100546, Human IGF1 Receptor ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman IGF1 Receptor ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human IGF1 Receptor in Human in Biofluids, Cell Culture Media samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Plasma, Cell culture supernatant, Serum,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:\u0026lt; 6 pg\/mL,\u003cbr\u003e\nRange:8.23 - 6000 pg\/mL,\u003cbr\u003e\nAssay Platform:Microplate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAbcam's IGF1 Receptor Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an\u003cbr\u003e\nin vitro\u003cbr\u003e\nenzyme-linked immunosorbent assay for the quantitative measurement of Human IGF1 Receptor in serum, plasma and cell culture supernatants.\u003cbr\u003e\nThis assay employs an antibody specific for Human IGF1 Receptor coated on a 96-well plate. Standards and samples are pipetted into the wells and IGF1 Receptor present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IGF1 Receptor antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGF1 Receptor bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.\u003cbr\u003e\nOptimization may be required with urine samples\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe IGF1 Receptor often referred to as IGF-1R is a transmembrane receptor protein composed of alpha and beta subunits. Studies indicate that the IGF1R protein is involved in mediating the effects of insulin-like growth factor 1 (IGF-1) and plays an important role in cellular signaling. It has a molecular weight of approximately 180 kDa and is widely expressed in various tissues with high concentrations in the liver muscle and brain. Being a tyrosine kinase receptor IGF1R plays an essential role in growth and metabolism.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nIGF1R contributes to several cellular processes including cell proliferation differentiation and survival. This receptor forms a complex upon ligand binding and undergoes autophosphorylation to activate intracellular signaling cascades. IGF1R activation recruits and phosphorylates insulin receptor substrates enabling the downstream signaling pathways that promote cell growth and survival. Its function in cellular responses makes it a focal point in understanding cell biology.\u003cbr\u003e\nPathways\u003cbr\u003e\nIGF1R holds a central position in the PI3K\/AKT and MAPK signaling cascades. These pathways play significant roles in cellular growth proliferation and survival. IGF1R phosphorylates various downstream effectors such as IRS-1 and Shc linking it to the activation of AKT and ERK respectively. Therefore it shares pathways with related proteins like the insulin receptor highlighting its importance in mediating similar biological responses.\u003cbr\u003e\nIGF1R has been implicated in cancers and diabetes. Overexpression of IGF1R has been observed in various malignancies including breast cancer where it relates to resistance in treatment and poor prognosis with cell lines like MCF-7 often being studied in this context. Additionally disruptions in IGF1R signaling link to insulin resistance an important feature of type 2 diabetes. These connections make IGF1R a potential therapeutic target with IGF1R inhibitors currently under exploration to address these health challenges.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843647033513,"sku":"ab100546","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab100546","provider":"Iright","version":"1.0","type":"link"}