{"product_id":"abcam-ab100593","title":"Abcam, ab100593, Human MICB ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman MICB ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human MICB in Human in Biofluids, Cell Culture Media samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Plasma, Cell culture supernatant, Serum,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:\u0026lt; 75 pg\/mL,\u003cbr\u003e\nRange:0.0686 - 50 ng\/mL,\u003cbr\u003e\nAssay Platform:Microplate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAbcam's MICB Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an\u003cbr\u003e\nin vitro\u003cbr\u003e\nenzyme linked immunosorbent assay for the quantitative measurement of Human MICB in serum, plasma, and cell culture supernatants.\u003cbr\u003e\nThis assay employs an antibody specific for Human MICB coated on a 96-well plate. Standards and samples are pipetted into the wells and MICB present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MICB antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MICB bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.\u003cbr\u003e\nOptimization may be required with urine samples.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nMICB also known as MHC class I polypeptide-related sequence B is a protein with a molecular weight of around 42 kDa. It anchors on cell surfaces and is widely expressed in epithelial cells fibroblasts and monocytes. MICB surfaces on stressed cells but remains minimal in normal conditions indicating its involvement in cell stress response. It is an activating ligand for NKG2D receptor on natural killer (NK) cells and certain T cells playing a major role in immune surveillance.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nMICB contributes to immune system functioning by presenting stress-induced antigens to immune cells. It does not belong to a complex but engages in interactions critical for activating cytotoxic responses. By binding to the NKG2D receptor it helps activate NK cells and certain T cell subsets which results in the elimination of damaged or transformed cells. This immune activation is important for the surveillance against cancer and infected cells maintaining tissue homeostasis.\u003cbr\u003e\nPathways\u003cbr\u003e\nMICB plays a role in the stress-induced NKG2D signaling pathway and is involved in the immune response pathway. It cooperates with proteins such as NKG2D and MICA another ligand of the NKG2D receptor to initiate immune cell-mediated cytotoxicity. These pathways activate NK cells and CD8+ T cells important for removing stressed or altered cells thereby influencing the body's defense mechanisms.\u003cbr\u003e\nMICB has connections to cancer and autoimmune disorders. Aberrant expression of MICB appears in tumors affecting tumor immune evasion and associates with autoimmune diseases influencing immune tolerance. Dysregulation of MICB activity can affect interactions with proteins like NKG2D altering immune responses and contributing to disease progression. Therefore understanding MICB's role is critical for developing therapeutic interventions targeting immune-related diseases.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843654176937,"sku":"ab100593","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab100593","provider":"Iright","version":"1.0","type":"link"}