{"product_id":"abcam-ab100658","title":"Abcam, ab100658, Human Elafin ELISA Kit (Skalp)","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman Elafin ELISA Kit (Skalp) is a sandwich ELISA designed to quantify Human Elafin (Skalp) with a sensitivity of 5 pg\/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 6.86 - 5000 pg\/mL\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Plasma, Cell culture supernatant, Serum,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 5 pg\/mL,\u003cbr\u003e\nRange:6.86 - 5000 pg\/mL,\u003cbr\u003e\nAssay Platform:Microplate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman Elafin ELISA Kit (Skalp) ab100658 is a sandwich ELISA to measure Human Elafin (Skalp) in serum, plasma, cell culture supernatant with a sensitivity of 5 pg\/ml.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nThis assay employs an antibody specific for Human Elafin coated on a 96-well plate. Standards and samples are pipetted into the wells and Elafin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Elafin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Elafin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.\u003cbr\u003e\nAssay Specificity\u003cbr\u003e\nOur ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details\u003cbr\u003e\nHuman Elafin ELISA Kit (Skalp) ab100658 protocol summary\u003cbr\u003e\n1. Add standard or sample to each well used. Incubate at room temperature\u003cbr\u003e\n2. Wash and add prepared biotinylated antibody to each well. Incubate at room temperature.\u003cbr\u003e\n3. Wash and add prepared streptavidin solution. Incubate at room temperature.\u003cbr\u003e\n4. Add TMB Development Solution to each well. Incubate at room temperature.\u003cbr\u003e\n5. Add Stop Solution to each well. Read at 450nm immediately.\u003cbr\u003e\nOptimization may be required with urine samples.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nElafin also known as ESI or ESI protein is a small serine protease inhibitor with a molecular mass of around 6 kDa. This protein produced by epithelial cells resides predominantly in the skin lungs and other mucosal surfaces. The Elafin protein plays an important mechanical role in inhibiting neutrophil elastase and proteinase 3. It contributes to the regulation of protease activity within tissues controlling inflammation and repair processes.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nElafin interacts intimately with protease inhibitors to guard tissues against excessive proteolytic damage. It does not typically form large complexes but functions as part of the innate immune defense. This protein helps to neutralize the action of neutrophil elastase which can help maintain the integrity of connective tissue extracellular matrix. Despite its standalone function Elafin's activity serves in tandem with other inhibitors in the protease-antiprotease balance.\u003cbr\u003e\nPathways\u003cbr\u003e\nElafin integrates prominently into the proteolytic and inflammatory pathways. It functions particularly in the inflammation and immune response pathways coordinating with proteins like secretory leukocyte protease inhibitor (SLPI) and alpha-1 antitrypsin. Elafin inhibits enzymes that degrade connective tissue components. Therefore it plays an important role in modulating inflammatory responses and tissue remodeling post-injury.\u003cbr\u003e\nConnections between Elafin and conditions such as chronic obstructive pulmonary disease (COPD) and psoriasis appear significant. These diseases relate to abnormal protease activity and chronic inflammation where Elafin's inhibitory function can be affected or diminished. Studies have shown that altered Elafin levels link with these diseases alongside proteins like neutrophil elastase and matrix metalloproteinases which contribute to tissue damage. Researchers continue to explore Elafin's therapeutic potential in managing such inflammatory conditions.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843643560105,"sku":"ab100658","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab100658","provider":"Iright","version":"1.0","type":"link"}