{"product_id":"abcam-ab102529","title":"Abcam, ab102529, Glucose 6 Phosphate Dehydrogenase Assay Kit (Colorimetric)","description":"\u003cp\u003eSize: 100Test\u003cbr\u003e\nGlucose 6 Phosphate Dehydrogenase Assay Kit (Colorimetric) (ab102529) is a simple, sensitive and rapid assay detects the activity of Glucose 6 Phosphate Dehydrogenase (G6PDH or G6PD) in a variety of samples. Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Urine, Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids,\u003cbr\u003e\nAssay type:Enzyme activity,\u003cbr\u003e\nSensitivity:\u0026gt; 0.04 mU\/well,\u003cbr\u003e\nAssay time:1h,\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nGlucose 6 Phosphate Dehydrogenase Assay Kit (Colorimetric) (ab102529) is a simple, sensitive and rapid assay detects the activity of Glucose 6 Phosphate Dehydrogenase (G6PDH or G6PD) in a variety of samples.\u003cbr\u003e\nIn the G6PD assay protocol, glucose 6 phosphate is oxidized by G6PD, and NADP is converted to NADPH. This leads to the chemically coupled conversion of a nearly colorless probe to an intensely colored product with an absorbance at 450nm.\u003cbr\u003e\nThe G6PD assay kit can detect as low as 0.04mU G6PD per well.\u003cbr\u003e\nG6PD assay protocol summary:\u003cbr\u003e\n- add samples and positive control to wells\u003cbr\u003e\n- add reaction mix\u003cbr\u003e\n- analyze on a microplate reader for 5-30 min\u003cbr\u003e\nThis product was previously called K757 Biovision Glucose-6-Phosphate Dehydrogenase Activity Colorimetric Assay Kit. Biovision was acquired by Abcam in 2021.\u003cbr\u003e\nThe Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nGlucose 6 Phosphate Dehydrogenase also known as G6PD or G6P dehydrogenase plays an important role in the pentose phosphate pathway catalyzing the conversion of glucose 6-phosphate to 6-phosphoglucono-Δ-lactone while producing NADPH from NADP+. G6PD with a molecular mass of approximately 59 kDa is expressed in various tissues with high levels found in red blood cells liver and adrenal glands. This enzyme is vital for protecting cells from oxidative damage by supplying reductive capacity.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nGlucose 6-phosphate dehydrogenase is essential for maintaining cellular redox balance especially in cells lacking mitochondria like red blood cells. It functions as part of a monomer which can dimerize depending on the cellular needs and conditions. The activity of G6PD directly affects the production of NADPH which is necessary for the biosynthesis of nucleic acids and lipids and for maintaining reduced glutathione levels therefore sustaining the antioxidant capacity of the cell.\u003cbr\u003e\nPathways\u003cbr\u003e\nGlucose 6-phosphate dehydrogenase is a pivotal enzyme in the oxidative phase of the pentose phosphate pathway which provides ribose 5-phosphate for nucleotide synthesis and NADPH for reductive biosynthetic reactions. G6PD connects with other enzymes in the pathway such as 6-phosphogluconate dehydrogenase contributing to the regulation of cellular metabolic needs. Its function is tightly linked to glucose metabolism and indirectly influences glycolytic processes.\u003cbr\u003e\nAlterations in glucose 6-phosphate dehydrogenase activity lead to conditions such as G6PD deficiency which can cause hemolytic anemia when individuals are exposed to certain drugs infections or foods. This enzyme's deficiency is one of the most common enzymatic disorders in humans and is triggered by genetic mutations affecting G6PD function. Moreover G6PD has implications in cancer biology as its activity influences the proliferation and survival of cancer cells alongside other proteins like TP53 and NF-κB which are involved in cellular stress responses.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843547386025,"sku":"ab102529","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab102529","provider":"Iright","version":"1.0","type":"link"}