{"product_id":"abcam-ab102536","title":"Abcam, ab102536, BCA Protein Assay Kit","description":"\u003cp\u003eSize: 1000Test\u003cbr\u003e\nBCA protein assay kit ab102536 is a simple, rapid, kit for determining the concentration of proteins in solution, within the range of 0.5-30 µg. - Detergent compatible (up to 5%) - Broad sample compatibility- including plasma, cell culture extracts, tissue extracts and serum - Readout on any colorimetric (562 nm) plate reader - Cited in over 160 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Plasma, Cell culture extracts, Tissue Extracts, Serum,\u003cbr\u003e\nAssay type:Quantitative,\u003cbr\u003e\nSensitivity:\u0026gt; 0.5 µg,\u003cbr\u003e\nRange:0.5 - 30 µg,\u003cbr\u003e\nAssay time:1h 30m\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nBCA Protein Assay Kit ab102536 provides a simple, rapid, detergent tolerant (up to 5%) procedure for determining the concentration of proteins in solution.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nThe BCA assay method utilizes a copper (Cu2+) salt which can be reduced to the cuprous state by protein(s).\u003cbr\u003e\nThe generated Cu+ ion forms an intensely colored complex with the bicinchoninic acid reagent with a very strong absorbance band centered at 562 nm.\u003cbr\u003e\nThe intensity of the blue complex is proportional to the amount of protein in the sample.\u003cbr\u003e\nThe BCA Protein Assay is suitable for measuring protein concentration in the range of 0.5-30 μg protein (0.01-0.6 mg\/ml). Support on how to determine protein concentration can be found\u003cbr\u003e\nGetting the best performance\u003cbr\u003e\nThe BCA assay protocol is very flexible. Both the incubation time and temperature can be varied over a rather wide range. Lower protein samples can be more easily quantified using higher temperatures and longer incubation times.\u003cbr\u003e\nWhen assaying protein in solutions containing detergent, best results are obtained by adding the same amount of detergent to the wells containing the protein standard.\u003cbr\u003e\nBCA assay protocol summary\u003cbr\u003e\n- mix Copper Reagent and BCA Reagent to make BCA assay working solution\u003cbr\u003e\n- dispense samples and standards into 96-well plate\u003cbr\u003e\n- add working solution to each well\u003cbr\u003e\n- shake gently and incubate for 30-90 minutes\u003cbr\u003e\n- measure absorbance in a plate reader at 562 nm\u003cbr\u003e\nOther notes\u003cbr\u003e\nThis product is manufactured by BioVision, an Abcam company and was previously called K812 BCA Protein Quantitation Kit. K812-1000 is the same size as the 1000 test size of ab102536.\u003cbr\u003e\nThe Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Please refer to protocols\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843596079273,"sku":"ab102536","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab102536","provider":"Iright","version":"1.0","type":"link"}