{"product_id":"abcam-ab102890","title":"Abcam, ab102890, HRP Conjugation Kit - Lightning-Link®","description":"\u003cp\u003eSize: 3 x 10µg \/ 100µg \/ 3 x 100µg \/ 1mg \/ 1 x 5mg \/ 5 x 1mg\u003cbr\u003e\nHRP Conjugation Kit (ab102890) offers several standout features: - Rapid Conjugation: achieve horseradish peroxidase (HRP) labeling in under 4 hours with just 30 seconds of hands-on time. - High Efficiency: ensures 100% antibody recovery, meaning no loss of valuable antibodies. - Versatility: suitable for conjugating antibodies, proteins, and peptides. HRP-labeled antibodies can be used immediately in applications such as Western blot, ELISA, and Immunohistochemistry (IHC) without further purification. - Confidence: cited in over 160 publications.\u003cbr\u003e\nProduct details:\u003cbr\u003e\nHRP Conjugation Kit \/ HRP Labeling Kit (ab102890) uses a simple and quick process for HRP labeling \/ conjugation of antibodies. It can also be used to conjugate other proteins or peptides.\u003cbr\u003e\nTo conjugate an antibody to HRP using this kit:\u003cbr\u003e\n- add modifier to antibody and incubate for 3 hrs\u003cbr\u003e\n- add quencher and incubate for 30 mins\u003cbr\u003e\nThe HRP conjugated antibody can be used immediately in WB, ELISA, IHC etc. No further purification is required and 100% of the antibody is recovered for use. Learn about buffer compatibility below . Custom size conjugation kits up to 100 mg are available on demand. Please contact us to discuss your requirements.\u003cbr\u003e\nThis product is manufactured by Expedeon, an Abcam company, and was previously called Lightning-Link\u003cbr\u003e\nHRP Labeling Kit. 701-0004 is the same as the 5 mg size. 701-0003 is the same as the 5 x 1 mg size. 701-0000 is the same as the 3 x 100 ug size. 701-0030 is the same as the 3 x 10 ug size. 701-0010 is the same as the 100 μg size. 701-0002 is the same as the 1 mg size.\u003cbr\u003e\nAmount and volume of antibody for conjugation to HRP\u003cbr\u003e\nKit size\u003cbr\u003e\nRecommended\u003cbr\u003e\namount of antibody\u003cbr\u003e\nMaximum\u003cbr\u003e\namount of antibody\u003cbr\u003e\nMaximum antibody\u003cbr\u003e\nvolume\u003cbr\u003e\n3 x 10 μg\u003cbr\u003e\n3 x 10 μg\u003cbr\u003e\n3 x 40 μg\u003cbr\u003e\n3 x 10 μL\u003cbr\u003e\n100 μg\u003cbr\u003e\n1 x 100 μg\u003cbr\u003e\n1 x 400 μg\u003cbr\u003e\n1 x 100 μL\u003cbr\u003e\n3 x 100 μg\u003cbr\u003e\n3 x 100 μg\u003cbr\u003e\n3 x 400 μg\u003cbr\u003e\n3 x 100 μL\u003cbr\u003e\n1 x 1 mg\u003cbr\u003e\n1 x 4 mg\u003cbr\u003e\n1 x 1 mL\u003cbr\u003e\n5 x 1 mg\u003cbr\u003e\n5 x 1 mg\u003cbr\u003e\n5 x 4 mg\u003cbr\u003e\n5 x 1 mL\u003cbr\u003e\n1 x 5 mg\u003cbr\u003e\n1 x 20 mg\u003cbr\u003e\n1 x 5 mL\u003cbr\u003e\nRecommended amount of antibody will give an HRP:antibody molar ratio of 4:1. Antibodies often also perform well at the maximum amount of antibody which is 1:1 molar ratio.\u003cbr\u003e\nIdeal antibody concentration is 1mg\/ml. 0.5 - 1 mg\/ml can be used if the maximum antibody volume is not exceeded. Antibodies \u0026gt; 4 mg\/ml or \u0026lt; 0.5 mg\/ml should be diluted \/concentrated.\u003cbr\u003e\nBuffer Requirements for Conjugation\u003cbr\u003e\nBuffer should be pH 6.5-8.5.\u003cbr\u003e\nBuffer constituent compatibility\u003cbr\u003e\nIf a concentration is shown, then the constituent should be no more than the concentration shown. If several constituents are close to the limit of acceptable concentration, then this can inhibit conjugation.\u003cbr\u003e\nCompatible buffer constituents\u003cbr\u003e\n50mM \/ 0.6% Tris\u003cbr\u003e\n0.1% BSA\u003cbr\u003e\n50% glycerol\u003cbr\u003e\n0.1% sodium azide\u003cbr\u003e\nPotassium phosphate\u003cbr\u003e\nSodium chloride\u003cbr\u003e\nSucrose\u003cbr\u003e\nSodium citrate\u003cbr\u003e\nTrehalose\u003cbr\u003e\nTris buffered saline is almost always ≤ 50 mM \/ 0.6%\u003cbr\u003e\nSodium azide irreversibly inhibits HRP; antibodies with azide should be purified before using the HRP conjugation kit.\u003cbr\u003e\nIncompatible buffer constituents\u003cbr\u003e\nThiomerosal\u003cbr\u003e\nProclin\u003cbr\u003e\nGlycine\u003cbr\u003e\nArginine\u003cbr\u003e\nGlutathione\u003cbr\u003e\nIf a constituent of the buffer containing your antibody or protein is not listed above, please contact us.\u003cbr\u003e\nOnly purified antibodies are suitable for use, ie. where other proteins, peptides, or amino acids are not present: antibodies in ascites fluid, serum or hybridoma culture media are incompatible.\u003cbr\u003e\nStoring and handling conjugation kits\u003cbr\u003e\nLyophilized Lightning-Link\u003cbr\u003e\ncomponents are hygroscopic.\u003cbr\u003e\nKits are intentionally shipped at ambient temperature with silica gel to avoid exposure to moisture. Upon receipt, store the kit frozen and protect from moisture. Before opening the outer container, allow the lyophilized components to reach room temperature to minimize condensation.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Ambient - Cannot Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847592136873,"sku":"ab102890","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab102890","provider":"Iright","version":"1.0","type":"link"}