{"product_id":"abcam-ab105135","title":"Abcam, ab105135, Aspartate Aminotransferase Activity Assay Kit","description":"\u003cp\u003eSize: 100Test \/ 2000Test\u003cbr\u003e\nAspartate Aminotransferase Activity Assay Kit \/ AST Assay Kit ab105135 provides a rapid, simple, sensitive and reliable assay for AST activity. - Includes positive control AST enzyme and glutamate standard for quantitation - Readout on any colorimetric (450 nm) plate reader - Cited in \u0026gt;80 publications - Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Plasma, Tissue Extracts, Cell culture supernatant, Serum, Saliva,\u003cbr\u003e\nAssay type:Enzyme activity,\u003cbr\u003e\nSensitivity:= 10 mU\/well,\u003cbr\u003e\nAssay time:1h,\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAspartate Aminotransferase Activity Assay Kit (AST assay kit) ab105135 provides a rapid, simple, sensitive and reliable assay for AST activity. Aspartate aminotransferase (AST) is also known as Aspartate transaminase and Glutamate-oxaloacetate transaminase (GOT).\u003cbr\u003e\nAST assay principle\u003cbr\u003e\nIn the AST assay, aspartate aminotransferase acts on aspartate and alpha-ketoglutarate, transferring an amino group from aspartate to α-ketoglutarate, and producing glutamate and oxaloacetate. Glutamate dehydrogenase then acts on the glutamate in an NAD-coupled reaction that produces a colorimetric readout (450 nm).\u003cbr\u003e\nAST Activity assay protocol summary\u003cbr\u003e\n- Add samples and standards to wells\u003cbr\u003e\n- Add reaction mix\u003cbr\u003e\n- Incubate at 37°C and analyze with microplate reader after 10 mins and then again after 60 mins\u003cbr\u003e\nHow other researchers are using ab105135\u003cbr\u003e\nAST Activity Assay Kit ab105135 has been used in a variety of sample types including:\u003cbr\u003e\nMouse cirrhosis-hepatocellular carcinoma\u003cbr\u003e\nDiabetic rat blood samples\u003cbr\u003e\nBlood sample from obese rats\u003cbr\u003e\nReferences: 1-Awad B et al. 2023, 2-Umar U et al. 2023, 3-Liao J et al. 2023\u003cbr\u003e\nRelated and recommended products\u003cbr\u003e\nFor fluorometric detection, we recommend Aspartate Aminotransferase Activity Assay Kit (Fluorometric)\u003cbr\u003e\nab138878\u003cbr\u003e\nAST Assay Kit \/ Aspartate Aminotransferase Activity Assay Kit ab105135 is often used with ALT Assay Kit \/ Alanine Transaminase Activity Assay Kit\u003cbr\u003e\nab105134\u003cbr\u003e\nas a marker for liver damage.\u003cbr\u003e\nThis product was previously called K753 Biovision Aspartate Aminotransferase (AST or SGOT) Activity Colorimetric Assay Kit. Biovision was acquired by Abcam in 2021.\u003cbr\u003e\nThe Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nAspartate Aminotransferase (AST) also known as aspartate transaminase or aspartate transferase is an enzyme important for amino acid metabolism. Mechanically it catalyzes the reversible transfer of an amino group from aspartate to α-ketoglutarate forming oxaloacetate and glutamate. AST has a molecular mass of approximately 92 kDa and is expressed in various tissues with high levels found in liver heart muscle and kidneys. Its presence in these tissues highlights its importance in cellular metabolic processes.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nAST facilitates the interconversion between aspartate and oxaloacetate playing a role in the amino acid and urea cycles. Though not part of a large complex AST works closely with similar enzymes such as alanine aminotransferase (ALT) to maintain amino acid balance and support energy production. This enzymatic activity is important in nitrogen metabolism and glutamate use which are necessary for synthesizing other essential molecules within the cell.\u003cbr\u003e\nPathways\u003cbr\u003e\nAST participates in the citric acid cycle and malate-aspartate shuttle enabling efficient energy production and NADH transport. The enzyme assists in converting oxaloacetate a pivotal intermediate in the citric acid cycle to keep the cycle active ensuring efficient cellular respiration. Additionally in the malate-aspartate shuttle AST works alongside malate dehydrogenase to facilitate the transfer of reducing equivalents across the mitochondrial membrane which is essential for ATP generation.\u003cbr\u003e\nElevated AST levels often indicate liver damage such as in hepatitis or cirrhosis reflecting its significant tissue expression. The enzyme is also linked to myocardial infarction as damaged heart muscle releases AST into the bloodstream. In these conditions AST serves as a biomarker for tissue damage often in conjunction with ALT levels allowing clinicians to assess the extent of liver or cardiac injury.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843540111529,"sku":"ab105135","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab105135","provider":"Iright","version":"1.0","type":"link"}