{"product_id":"abcam-ab107921","title":"Abcam, ab107921, Proteasome Activity Assay Kit","description":"\u003cp\u003eSize: 100Test\u003cbr\u003e\nProteasome Activity Assay Kit ab107921 is based on a Succ-LLVY-AMC substrate which is cleaved by proteasome activity, releasing fluorescent AMC. Readout on any fluorometric (Ex\/Em 350\/440 nm) plate reader. - Includes proteasome inhibitor control, proteasome positive control, and standard to allow proteasome activity quantitation - Cited in \u0026gt; 80 publications - Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Fluorescent,\u003cbr\u003e\nSample types:Cell Lysate,\u003cbr\u003e\nAssay type:Enzyme activity,\u003cbr\u003e\nAssay time:1h,\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nProteasome Activity Assay Kit ab107921 is a simple and reliable assay to quantify proteasome activity.\u003cbr\u003e\nProteasome activity assay principle\u003cbr\u003e\nThe 20S proteasome assembly is the functional protease structure in the proteasome, with chymotrypsin-like, trypsin-like and caspase-like protease activities. The assay is based on measuring the chymotrypsin-like activity.\u003cbr\u003e\nThe assay uses an AMC-tagged peptide substrate (Proteasome Substrate (Succ-LLVY-AMC in DMSO), which releases free, fluorescent AMC (Ex\/Em 350\/440 nm) in the presence of proteolytic activity.\u003cbr\u003e\nThe kit also includes a positive control (Jurkat cell lysate with significant proteasome activity), and a specific proteasome inhibitor MG-132 which suppresses all proteolytic activity due to proteasomes. This permits differentiation of proteasome activity from other protease activity which may be present in samples.\u003cbr\u003e\nProteasome activity assay protocol summary\u003cbr\u003e\n- add samples, standards and positive control to wells\u003cbr\u003e\n- add proteasome inhibitor to half of sample and positive control wells\u003cbr\u003e\n- add proteasome substrate to sample and control wells\u003cbr\u003e\n- incubate for 20 min\u003cbr\u003e\n- analyze with microplate reader, incubate for further 30 min, analyze again\u003cbr\u003e\nHow other researchers are using Proteasome Activity Assay Kit ab107921\u003cbr\u003e\nab107921 has been used in a variety of sample types including:\u003cbr\u003e\n- Mouse ovarian surface epithelial cell lines (1)\u003cbr\u003e\n- Mouse intestine samples (2)\u003cbr\u003e\n- Mouse brain lysates (3)\u003cbr\u003e\nReferences: 1-Tailor D et al. 2023; 2-Hurley M et al. 2023; 3-Vulin M et al. 2023\u003cbr\u003e\nRelated and recommended products\u003cbr\u003e\nProteasome 20S Activity Assay Kit (Fluorometric)\u003cbr\u003e\nab112154\u003cbr\u003e\nuses an alternative fluorophore and a similar principle to this assay, with an LLVY proteasome substrate.\u003cbr\u003e\nThis product was previously called K245 Biovision Proteasome Activity Fluorometric Assay Kit. Biovision was acquired by Abcam in 2021.\u003cbr\u003e\nThe Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nProteasomes are large protein complexes responsible for degrading ubiquitinated proteins into peptides. This process is essential for maintaining protein homeostasis within the cell. The proteasome often referred to as the 26S proteasome consists of a core particle with a mass of roughly 700 kDa and a regulatory particle. They are expressed in the cytoplasm and nucleus of eukaryotic cells where they perform the bulk of cellular protein degradation. Other names for the proteasome include multicatalytic proteinase complex and prosome.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe ubiquitin-proteasome system orchestrates the controlled degradation of unneeded or damaged proteins regulating numerous cellular mechanisms. The proteasome forms a complex with ubiquitin chains tagged to substrate proteins guiding them for degradation. Proteasomal activity ensures turnover of proteins involved in cell cycle progression signal transduction and immune responses. By controlling protein levels proteasome function influences many biological outcomes and maintains cellular equilibrium.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe proteasome's function integrates deeply into the ubiquitin-proteasome pathway critical for protein catabolism and cellular control processes. Another significant pathway is the regulation of apoptosis where the proteasome controls the levels of proteins like p53 an important regulator of cell death. In these pathways the proteasome collaborates with other proteins such as ubiquitin ligases playing role in fine-tuning the cellular processes involved in survival and programmed cell death.\u003cbr\u003e\nProteasome dysfunction contributes to neurodegenerative diseases like Parkinson's and Alzheimer's. Disruption in proteasomal activity leads to aggregation of damaged proteins which can have pathological consequences. Cancer is another area where proteasomes play an important role; certain cancers show dysregulated proteasomal activity resulting in abnormal degradation of tumor suppressor proteins like p53. Understanding proteasome markers helps in diagnosing and evaluating these diseases linking proteasomes to both prognosis and potential therapeutic interventions.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843543257257,"sku":"ab107921","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab107921","provider":"Iright","version":"1.0","type":"link"}