{"product_id":"abcam-ab108666","title":"Abcam, ab108666, Testosterone ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nTestosterone ELISA kit is a competitive ELISA designed to quantify human testosterone in serum, plasma (citrate, heparin), and urine with a sensitivity of 0.07 ng\/mL. - Wide dynamic range – quantifies 0.2–16 ng\/mL - Designed to measure bound and free testosterone - High recovery rate – achieves 105.5% general recovery - Cited in over 55 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Citrate plasma, Heparin Plasma, Serum, Urine,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Competitive,\u003cbr\u003e\nSensitivity:= 0.07 ng\/mL,\u003cbr\u003e\nRange:0.2 - 16 ng\/mL,\u003cbr\u003e\nAssay Platform:Microplate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nTestosterone ELISA Kit ab108666 is a competitive ELISA kit to measure human Testosterone (17β-Hydroxy-4-androstene-3-one) in serum, plasma (citrate, heparin), and urine with a sensitivity of 0.07 ng\/mL.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nTestosterone ELISA Kit ab108666 is a competitive ELISA. There are 2 types of competitive ELISAs:\u003cbr\u003e\nantigen down\u003cbr\u003e\n: The sample antigen competes with a reference antigen for binding to a specific amount of labeled antibody. The reference antigen is pre-coated on a multi-well plate. The sample is added to the plate wells with a labeled antibody. Depending on the amount of antigen in the sample, more or fewer free antibodies will be available to bind the reference antigen. This means that the more of the antigen there is in the sample, the less reference antigen will be detected and the weaker the signal will be.\u003cbr\u003e\nantibody down\u003cbr\u003e\n: the labeled antigen and the sample antigen (unlabeled) compete for binding to the primary antibody, which is immobilized on the plate. The lower the amount of antigen in the sample, the stronger the signal due to more labelled antigen in the well.\u003cbr\u003e\nAssay specificity\u003cbr\u003e\nOur ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility.\u003cbr\u003e\nTestosterone ELISA Kit ab108666 protocol summary\u003cbr\u003e\nAdd standards or samples to the wells, incubate 2 hrs\u003cbr\u003e\nWash, then add 50 µl of Biotinylated Antibody per well. Incubate 1 hr\u003cbr\u003e\nWash, then add 50 µl of SP Conjugate per well. Incubate 30 min\u003cbr\u003e\nWash, then add 50 µl of Chromogen Substrate per well. Incubate 20 min\u003cbr\u003e\nAdd 50 µl of Stop Solution per well. Read at 450 nm immediately\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nTestosterone also known as 17β-hydroxyandrost-4-en-3-one is a steroid hormone with a molecular mass of approximately 288.42 Da. It is primarily synthesized in the testes but also in smaller amounts in the ovaries and adrenal glands. Mechanically testosterone operates by binding to the androgen receptor a member of the nuclear receptor family which then modulates gene expression. Its synthesis is stimulated by luteinizing hormone (LH) which is released from the anterior pituitary gland.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nTestosterone plays an important role in the development of male secondary sexual characteristics such as increased muscle and bone mass and the growth of body hair. It's also part of the steroid hormone biosynthesis pathway influencing the production of estradiol through the aromatase enzyme. Testosterone contributes to the maintenance of reproductive tissues and is important for the modulation of mood and cognitive functions.\u003cbr\u003e\nPathways\u003cbr\u003e\nTestosterone is integral to the HPG (hypothalamic-pituitary-gonadal) axis where it exerts feedback control by inhibiting the secretion of gonadotropin-releasing hormone (GnRH) and LH. Moreover testosterone is essential in the androgen receptor signaling pathway. This pathway involves other proteins like dihydrotestosterone (DHT) a potent androgen converted from testosterone via the 5α-reductase enzyme.\u003cbr\u003e\nLow levels of testosterone commonly known as 'Low T' are linked to conditions like hypogonadism and certain metabolic syndromes. Testosterone levels can influence the risk of osteoporosis as sustained low levels reduce bone density. Additionally increased or decreased testosterone levels can pose risks to cardiovascular health with the target interacting with proteins such as sex hormone-binding globulin (SHBG) in these contexts.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843542765737,"sku":"ab108666","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab108666","provider":"Iright","version":"1.0","type":"link"}