{"product_id":"abcam-ab108698","title":"Abcam, ab108698, Human Ferritin ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman Ferritin ELISA Kit is a sandwich ELISA designed to quantify Human Ferritin with a sensitivity of 0.53 ng\/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Cited in over 10 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Citrate plasma, Heparin Plasma, Serum,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 0.53 ng\/mL,\u003cbr\u003e\nAssay Platform:Microplate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman Ferritin ELISA Kit ab108698 is a sandwich ELISA to measure Human Ferritin in serum, citrate plasma, heparin plasma with a sensitivity of 0.53 ng\/ml.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nA 96-well plate has been precoated with anti-Ferritin IgG antibodies. Samples, standards and the Ferritin-HRP conjugate are added to the wells, where Ferritin in the sample and standards binds to the precoated antibody and added Ferritin-HRP conjugate binds to this antibody-AFP complex. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is directly proportional to the amount of Ferritin in the sample and the intensity is measured at 450 nm.\u003cbr\u003e\nAssay Specificity\u003cbr\u003e\nOur ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details\u003cbr\u003e\nHuman Ferritin ELISA Kit ab108698 protocol summary\u003cbr\u003e\n1. Add samples, standards and controls to wells used.\u003cbr\u003e\n2. Add prepared labeled HRPConjugate to each well. Incubate at room temperature.\u003cbr\u003e\n3. After washing, add TMB substrate solution to each well. Incubate at room temperature.\u003cbr\u003e\n4. Add Stop Solution to each well. Read immediately.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843546206377,"sku":"ab108698","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab108698","provider":"Iright","version":"1.0","type":"link"}