{"product_id":"abcam-ab108715","title":"Abcam, ab108715, Human Anti-Candida albicans IgG ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman Anti-Candida albicans IgG ELISA Kit is an indirect ELISA for the qualitative detection of IgG class antibodies against Candida albicans in human plasma and serum samples. - Colorimetric readout - 450 nm - Works on any standard plate reader - Easy results interpretation - Cut-off, positive and negative controls included\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Citrate plasma, Heparin Plasma, Serum,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Indirect,\u003cbr\u003e\nAssay Platform:Microplate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman Anti-Candida albicans IgG ELISA Kit ab108715 is an indirect ELISA kit for the qualitative measurement of Human Anti-Candida albicans IgG in Citrate plasma, Heparin Plasma, Serum.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nA 96-well plate has been precoated with Candida albicans antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Candida albicans-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Candida albicans IgG sample captured in plate.\u003cbr\u003e\nAssay protocol summary\u003cbr\u003e\nHuman Anti-Candida albicans IgG ELISA Kit ab108715 protocol summary:\u003cbr\u003e\n1. Add samples and controls to wells used.Incubate at 37°C.\u003cbr\u003e\n2. Wash each well and add prepared labeled HRP-Conjugate. Incubate at room temperature.\u003cbr\u003e\n3. After washing, add TMB substrate solution to each well. Incubate at room temperature.\u003cbr\u003e\n4. Add Stop Solution to each well. Read immediately.\u003cbr\u003e\nGetting the best performance\u003cbr\u003e\nASSAY SPECIFICITY\u003cbr\u003e\nThe specificity is \u0026gt; 95 % and is defined as the probability of the assay scoring negative in the absence of the specific analyte.\u003cbr\u003e\nASSAY SENSITIVITY\u003cbr\u003e\nThe sensitivity is 93 % and is defined as the probability of the assay scoring positive in the presence of the specific analyte.\u003cbr\u003e\nINTERFERENCES\u003cbr\u003e\nInterferences with hemolytic, lipemic or icteric sera are not observed up to a concentration of 10 mg\/mL hemoglobin, 5 mg\/mL triglycerides and 0.2 mg\/mL bilirubin.\u003cbr\u003e\nRelated and recommended products\u003cbr\u003e\nHuman Anti-Candida albicans IgG ELISA Kit ab108715 is commonly used to study candida albicans and infections.\u003cbr\u003e\nOther ELISA kits for measuring immune responses to Candida albicans are:\u003cbr\u003e\n- Human Anti-Candida albicans IgA ELISA kit\u003cbr\u003e\nab108714\u003cbr\u003e\n- Human Anti-Candida albicans IgM ELISA kit\u003cbr\u003e\nab108716\u003cbr\u003e\n-Human Calprotectin ELISA kit (S100A8\/S100A9)\u003cbr\u003e\nab267628\u003cbr\u003e\nHuman Anti-Candida albicans IgG ELISA Kit ab108715 is an indirect ELISA kit for the qualitative measurement of Human Anti-Candida albicans IgG in Citrate plasma, Heparin Plasma, Serum .\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nA 96-well plate has been precoated with Candida albicans antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Candida albicans-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Candida albicans IgG sample captured in plate.\u003cbr\u003e\nAssay specificity\u003cbr\u003e\nThe specificity is \u0026gt; 95 % and is defined as the probability of the assay scoring negative in the absence of the specific analyte.\u003cbr\u003e\nThe sensitivity is 93 % and is defined as the probability of the assay scoring positive in the presence of the specific analyte.\u003cbr\u003e\nInterferences with hemolytic, lipemic or icteric sera are not observed up to a concentration of 10 mg\/mL hemoglobin, 5 mg\/mL triglycerides and 0.2 mg\/mL bilirubin.\u003cbr\u003e\nHuman Anti-Candida albicans IgG ELISA Kit ab108715 protocol summary\u003cbr\u003e\n1. Add samples and controls to wells used. Incubate at 37єC\u003cbr\u003e\n2. Wash each well and add prepared labeled HRP-Conjugate. Incubate at room temperature\u003cbr\u003e\n3. After washing, add TMB substrate solution to each well. Incubate at room temperature\u003cbr\u003e\n4. Add Stop Solution to each well. Read immediately\u003cbr\u003e\nRelated and recommended products\u003cbr\u003e\nHuman Anti-Candida albicans IgG ELISA Kit ab108715 is commonly used to study candida albicans and infections.\u003cbr\u003e\nOther ELISA kits for measuring immune responses to Candida albicans are:\u003cbr\u003e\n- Human Anti-Candida albicans IgA ELISA kit\u003cbr\u003e\nab108714\u003cbr\u003e\n- Human Anti-Candida albicans IgM ELISA kit\u003cbr\u003e\nab108716\u003cbr\u003e\n- Human Calprotectin ELISA kit (S100A8\/S100A9)\u003cbr\u003e\nab267628\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843558756521,"sku":"ab108715","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab108715","provider":"Iright","version":"1.0","type":"link"}