{"product_id":"abcam-ab108792","title":"Abcam, ab108792, Mouse Albumin ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nMouse Albumin ELISA Kit is a Sandwich ELISA used to quantify Mouse Albumin with a sensitivity of 0.45 ng\/ml - Colorimetric ELISA kit - 450 nm readout. Works on any standard plate reader - Wide dynamic range – 0.781 - 200 ng\/ml - Cited in over 95 citations\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Urine, Plasma, Cell culture supernatant, Serum, Cell Lysate, Tissue,\u003cbr\u003e\nReacts with:Mouse,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:\u0026gt; 0.45 ng\/mL,\u003cbr\u003e\nRange:0.781 - 200 ng\/mL,\u003cbr\u003e\nAssay Platform:Microplate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nMouse Albumin ELISA kit (ab108792) is a sandwich ELISA kit designed for the quantitative measurement of mouse Albumin levels in plasma, serum, urine, cell culture supernatant, cell lysate and tissue samples.\u003cbr\u003e\nAn Albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Albumin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Albumin captured in plate.\u003cbr\u003e\nThe entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.\u003cbr\u003e\nThe entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.\u003cbr\u003e\nMouse Albumin ELISA Kit ab108792 is a competitive ELISA to measure mouse albumin in urine and cell culture supernatants with a sensitivity of \u0026gt; 0.45 ng\/mL.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nAn Albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Albumin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Albumin captured in plate.\u003cbr\u003e\nAssay specificity\u003cbr\u003e\nThe assay has no reactivity with canine, bovine, equine, monkey, human, swine, rabbit albumin.\u003cbr\u003e\nthe reactivity with rat albumin is \u0026lt;10%\u003cbr\u003e\nMouse Albumin ELISA Kit ab108792 protocol summary\u003cbr\u003e\n1. Add 25 µl of Standard or Sample and 25 µl of Biotinylated Protein per well. Incubate 1 hour.\u003cbr\u003e\n2. Wash, then add 50 µl of SP Conjugate per well. Incubate 30 minutes.\u003cbr\u003e\n3. Wash, then add 50 µl of Chromogen Substrate per well. Incubate 20 minutes.\u003cbr\u003e\n4. Add 50 µl of Stop Solution per well. Read at 450 nm immediately.\u003cbr\u003e\nHow other researchers are using Mouse Albumin ELISA kit ab108792\u003cbr\u003e\nMouse Albumin ELISA kit ab108792 has been used to measure the Albumin Creatinin Ratio (ACR) in mice with polycystic kidney disease\u003cbr\u003e\nand to assess hepatocyte derived organoids\u003cbr\u003e\nReferences:\u003cbr\u003e\nMS Tham etal., 2024.PMID:38191531,\u003cbr\u003e\nJ Liang et al., 2023, PMID:37907970\u003cbr\u003e\nGet results in 90 minutes our SimpleStep ELISA® kit:\u003cbr\u003e\n- Mouse albumin ELISA kit\u003cbr\u003e\nab207620\u003cbr\u003e\nMouse Albumin ELISA kit\u003cbr\u003e\nab109792\u003cbr\u003e\nis commonly used by researchers to measure liver or kidney function.\u003cbr\u003e\nLiver function ELISA assays include Mouse ALT ELISA kit\u003cbr\u003e\nab282882\u003cbr\u003e\nand Mouse AST ELISA Kit\u003cbr\u003e\nab263882\u003cbr\u003e\nThese ELISA kits are also available for Rat:\u003cbr\u003e\n- Rat Albumin ELISA kit\u003cbr\u003e\nab235642\u003cbr\u003e\n- Rat ALT ELISA kit abab234579\u003cbr\u003e\n- Rat AST ELISA kit\u003cbr\u003e\nab263883\u003cbr\u003e\nKidney function marker assays\u003cbr\u003e\n- Creatinine Assay Kit\u003cbr\u003e\nab65340\u003cbr\u003e\n– suitable for use as a serum creatinine assay and a urine creatinine assay\u003cbr\u003e\n- Albumin (BCG) Assay Kit (Colorimetric)\u003cbr\u003e\nab235628\u003cbr\u003e\n– suitable for use with serum\u003cbr\u003e\n- Urea Assay Kit\u003cbr\u003e\nab83362\u003cbr\u003e\n– suitable for use with serum\/plasma and urine, and used as a blood urea nitrogen assay.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nAlbumin also known as ALB is a major plasma protein with a molecular mass of approximately 66.5 kDa. It predominantly gets synthesized in the liver and found abundantly in blood plasma. Albumin serves multiple functions including maintaining osmotic pressure and acting as a carrier protein for various endogenous substances like hormones fatty acids and bilirubin.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nAlbumin plays a critical role in transporting small molecules like metabolites and drugs through the circulatory system. It is not a part of any complex but functions independently to bind transport and release various ligands. Albumin's affinity for hydrophobic molecules makes it essential for they to be solubilized in blood for proper metabolism and excretion.\u003cbr\u003e\nPathways\u003cbr\u003e\nMore than five binding sites on albumin facilitate its role in the fatty acid metabolism and renin-angiotensin system pathways. In fatty acid metabolism albumin interacts with transport proteins carrying fatty acids to tissues for energy production. Additionally during the renin-angiotensin system albumin modulates blood pressure through its interaction with angiotensin II which can influence vasoconstriction.\u003cbr\u003e\nResearchers link albumin with nephrotic syndrome and liver cirrhosis. In nephrotic syndrome low levels of albumin (albumin low) can cause edema due to imbalanced osmotic pressure. In liver cirrhosis a reduction in albumin synthesis signifies liver damage. Albumin's interaction with proteins like angiotensin II correlates with complications such as hypertension further establishing the protein's role in health and disease contexts.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843539488937,"sku":"ab108792","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab108792","provider":"Iright","version":"1.0","type":"link"}