{"product_id":"abcam-ab108816","title":"Abcam, ab108816, Rat BNP 45 ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nRat BNP 45 ELISA Kit is a sandwich ELISA designed to quantify Rat BNP 45 with a sensitivity of 7.9 pg\/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 15.625 - 1000 pg\/mL - Cited in over 20 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Plasma, Serum,\u003cbr\u003e\nReacts with:Rat,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 7.9 pg\/mL,\u003cbr\u003e\nRange:15.625 - 1000 pg\/mL,\u003cbr\u003e\nAssay Platform:Microplate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nRat BNP 45 ELISA Kit ab108816 is a sandwich ELISA to measure Rat BNP 45 in serum and plasma with a sensitivity of 7.9 pg\/ml.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nA BNP 45 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a BNP 45 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of BNP 45 captured in plate.\u003cbr\u003e\nThe entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.\u003cbr\u003e\nAssay Specificity\u003cbr\u003e\nOur ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details\u003cbr\u003e\nRat BNP 45 ELISA Kit ab108816 protocol summary\u003cbr\u003e\n1. Add standard or sample to appropriate wells. Incubate at room temperature\u003cbr\u003e\n2. Wash and add prepared biotin antibody to each well. Incubate at room temperature.\u003cbr\u003e\n3. Wash and add prepared Streptavidin-Peroxidase Conjugate. Incubate at room temperature\u003cbr\u003e\n4. Add Chromogen Substrate to each well. Incubate at room temperature\u003cbr\u003e\n5. Add Stop Solution to each well. Read at 450 nm immediately.\u003cbr\u003e\nThere is significant cross-reactivity between BNP32 and BNP45.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843547123881,"sku":"ab108816","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab108816","provider":"Iright","version":"1.0","type":"link"}