{"product_id":"abcam-ab108854","title":"Abcam, ab108854, Human Alpha 1 acid Glycoprotein \/ AGP ELISA Kit (AGP)","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman Alpha 1 acid Glycoprotein \/ AGP ELISA Kit (AGP) is a competitive ELISA designed to quantify Human Alpha 1 acid Glycoprotein (AGP) with a sensitivity of 0.1 µg\/mL - Colorimetric competitive ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 0.25 - 2 µg\/ml\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Plasma, Serum,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Competitive,\u003cbr\u003e\nSensitivity:= 0.1 µg\/mL,\u003cbr\u003e\nRange:0.25 - 2 µg\/mL,\u003cbr\u003e\nAssay time:3h,\u003cbr\u003e\nAssay Platform:Microplate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAbcam's Alpha 1 acid Glycoprotein \/ AGP Human\u003cbr\u003e\nin vitro\u003cbr\u003e\ncompetitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitive measurement of Alpha 1 acid Glycoprotein \/ AGP in plasma and serum.\u003cbr\u003e\nAn Alpha 1 acid Glycoprotein \/ AGP specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Alpha 1 acid Glycoprotein \/ AGP is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Alpha 1 acid Glycoprotein \/ AGP captured in plate.\u003cbr\u003e\nThe entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nAlpha 1 Acid Glycoprotein also known as AGP or Alpha 1 Glycoprotein is a plasma protein with a molecular weight of approximately 41 to 43 kDa. This protein circulates primarily in the bloodstream and is produced mainly by the liver. AGP is known for its highly glycosylated structure carrying several branched oligosaccharide chains. It exhibits a significant capacity to bind various endogenous and exogenous lipophilic substances influencing their transport and distribution in the body.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nAGP plays a role in modulating the immune response and maintaining homeostasis. As an acute-phase protein its levels rise significantly during inflammation infection and trauma where it acts to modulate the immune system. AGP does not exist as part of a larger protein complex but interacts with plasma lipoproteins and other glycoproteins aiding in its function of maintaining vascular integrity during acute-phase reactions.\u003cbr\u003e\nPathways\u003cbr\u003e\nAlpha 1 Glycoprotein serves as an important component in the inflammatory response and drug-binding pathways. In the inflammatory pathway AGP interacts with cytokines and acute-phase reactants adjusting the body’s inflammatory processes. It is also involved in the binding and distribution of drugs interacting with albumin and other transport proteins to influence the pharmacokinetics of many medications.\u003cbr\u003e\nAGP levels change in response to conditions like rheumatoid arthritis and liver diseases. In rheumatoid arthritis its increased levels correlate with disease activity and inflammation. In liver diseases the production and plasma concentration of AGP can reflect hepatic synthesis capability. AGP's interaction with cytokines in these conditions often links it to elevated levels of other acute-phase proteins like C-reactive protein reflecting its participation in the pathophysiological processes of inflammation.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843620556969,"sku":"ab108854","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab108854","provider":"Iright","version":"1.0","type":"link"}