{"product_id":"abcam-ab108862","title":"Abcam, ab108862, Human Heat Shock Protein 27 ELISA Kit (HSP27)","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman Heat Shock Protein 27 ELISA Kit (HSP27) is a Sandwich (quantitative) ELISA for the measurement of Human Heat Shock Protein 27 (HSP27) in Human in Biofluids, Cell\/Tissue Extracts samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Plasma, Tissue, Milk, Serum, Cell Lysate,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:\u0026gt; 0.16 ng\/mL,\u003cbr\u003e\nRange:1.25 - 20 ng\/mL,\u003cbr\u003e\nAssay time:5h,\u003cbr\u003e\nAssay Platform:Microplate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAbcam's Heat Shock Protein 27 (HSP27) Human\u003cbr\u003e\nin vitro\u003cbr\u003e\nELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of HSP27 levels in cell culture samples, milk, serum, plasma and tissue extract.\u003cbr\u003e\nA HSP27 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a HSP27 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of HSP27 captured in plate.\u003cbr\u003e\nGet results in 90 minutes with Human Heat Shock Protein 27 ELISA Kit (HSP27) (\u003cbr\u003e\nab193757\u003cbr\u003e\n) from our SimpleStep ELISA\u003cbr\u003e\nrange.\u003cbr\u003e\nThe entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nHeat Shock Protein 27 (HSP27) also known as HSPB1 is a small heat shock protein with a molecular weight of approximately 27 kDa. It is expressed in various tissues including muscle heart and brain and responds to stress stimuli such as heat shock oxidative stress and inflammation. HSP27 functions as a molecular chaperone helping to stabilize unfolded proteins and prevent aggregation. Its expression increases significantly in cells undergoing stress playing a protective role in maintaining protein homeostasis.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe small HSP protein functions in cellular processes like apoptosis cytoskeletal organization and protein folding. It does not act alone but often interacts with other proteins to form complexes that perform specific functions efficiently. HSP27 can bind to actin filaments influencing cell shape and motility. It also plays a role in preventing cell death by inhibiting apoptosome formation and modulating caspase activation. The dual role of protecting cells and modulating cell growth makes HSP27 an important tool for cells under stress.\u003cbr\u003e\nPathways\u003cbr\u003e\nHSP27 interacts with the mitogen-activated protein kinase (MAPK) and PI3K\/AKT signaling pathways. These pathways regulate cell survival growth and differentiation. Within these pathways HSP27 is linked to proteins such as Akt and MAPK which are critical for relaying survival and growth signals. By interacting with these molecules HSP27 helps to mediate the cell's response to external stressors and promotes cell survival under adverse conditions.\u003cbr\u003e\nHSP27 is significant in cancer and neurodegenerative diseases. Its overexpression often occurs in various cancers including breast and prostate cancer where it contributes to tumor growth and resistance to chemotherapy. In neurodegenerative diseases like Alzheimer's disease altered expression and functioning of HSP27 can lead to aggregation of proteins such as tau contributing to disease pathology. The link between HSP27 and these proteins shows its potential as a therapeutic target for modulating disease progression.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843641364649,"sku":"ab108862","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab108862","provider":"Iright","version":"1.0","type":"link"}