{"product_id":"abcam-ab108891","title":"Abcam, ab108891, Human PAI1 ELISA Kit (SERPINE1)","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman PAI1 ELISA Kit (SERPINE1) is a Sandwich (quantitative) ELISA for the measurement of Human PAI1 (SERPINE1) in Human in Cell\/Tissue Extracts, Cell Culture Media, Biofluids samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Cell Lysate, Cell culture supernatant, Cerebral Spinal Fluid, Plasma, Serum, Tissue,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 19 pg\/mL,\u003cbr\u003e\nRange:0.15 - 1.25 ng\/mL,\u003cbr\u003e\nAssay time:4h,\u003cbr\u003e\nAssay Platform:Microplate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAbcam's PAI1 (SERPINE1) Human\u003cbr\u003e\nin vitro\u003cbr\u003e\nELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of PAI1 concentrations in Human plasma, serum, tissue extracts and cell culture supernatants.\u003cbr\u003e\nA PAI1 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a PAI1 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of PAI1 captured in plate.\u003cbr\u003e\nThis assay recognizes both natural and recombinant Human PAI-1.\u003cbr\u003e\nThe entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843626389673,"sku":"ab108891","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab108891","provider":"Iright","version":"1.0","type":"link"}