{"product_id":"abcam-ab109714","title":"Abcam, ab109714, ATP synthase Activity Assay Kit (Colorimetric)","description":"\u003cp\u003eSize: 96Test\u003cbr\u003e\nATP synthase Activity Assay Kit (Colorimetric) ab109714 is used to determine the activity of ATP synthase (Complex V) in a human or rat sample.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Cell culture extracts, Tissue, Suspension cells, Tissue Extracts, Purified mitochondria, Cell Lysate,\u003cbr\u003e\nReacts with:Rat, Cow, Human,\u003cbr\u003e\nAssay type:Enzyme activity,\u003cbr\u003e\nAssay time:6h,\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nATP synthase Activity Assay Kit (Colorimetric) ab109714 is used to determine the activity of ATP synthase (Complex V) in a human or rat sample.\u003cbr\u003e\nThe ATP synthase enzyme is immunocaptured within the wells of the microplate and the enzyme activity is measured by monitoring the decrease in absorbance at 340 nm. The conversion of ATP to ADP by ATP synthase is coupled to the oxidation reaction of NADH to NAD\u003cbr\u003e\nwith a reduction in absorbance at 340 nm.\u003cbr\u003e\nOther Notes\u003cbr\u003e\nThis product was previously called ATP synthase Enzyme Activity Microplate Assay Kit\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Dry Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nATP synthase also known as Complex V or F1F0-ATPase catalyzes the synthesis of ATP from ADP and inorganic phosphate. The multisubunit enzyme has a mass of approximately 500 kDa and resides in the inner mitochondrial membrane. It also appears in bacterial plasma membranes and the thylakoid membrane of chloroplasts. ATP synthase operates through a rotary mechanism converting a proton gradient into mechanical energy which then converts into chemical energy in the form of ATP.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nATP synthase functions as a critical component of cellular energy production. As part of the ATP synthase complex it carries out ATP synthesis essential for numerous biological processes. By utilizing a proton-motive force it enables the regeneration of mitochondrial ATP integral for maintaining cellular energy homeostasis. The enzyme's efficient ATP activity highlights its role in ensuring sustained energy supply within cells.\u003cbr\u003e\nPathways\u003cbr\u003e\nATP synthase plays an important role in the oxidative phosphorylation pathway. This pathway occurs in mitochondria where the enzyme functions alongside other proteins such as NADH dehydrogenase and cytochrome c oxidase. These proteins forming the electron transport chain contribute to establishing the proton gradient required for ATP synthase activity. Additionally ATP synthase activity is closely linked to the citric acid cycle providing the necessary gradient for ATP production.\u003cbr\u003e\nATP synthase relates to conditions such as mitochondrial diseases and neurodegenerative disorders. Dysfunctional mitochondrial ATP synthase can lead to energy deficiency and mitochondrial myopathies. It also has connections to proteins like mitochondrial DNA mutations which impact ATP synthase function and cause cellular energy deficits. Furthermore impaired ATP synthase activity can contribute to conditions like Parkinson's disease where energy metabolism disruptions play a role.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843540045993,"sku":"ab109714","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab109714","provider":"Iright","version":"1.0","type":"link"}