{"product_id":"abcam-ab109911","title":"Abcam, ab109911, Complex IV Rodent Enzyme Activity Microplate Assay Kit","description":"\u003cp\u003eSize: 96Test\u003cbr\u003e\nComplex IV Rodent Enzyme Activity Microplate Assay Kit (ab109911) is used to determine the activity of cytochrome c oxidase in mouse samples. The assay is based on antibody-based capture of Complex IV on the plate, followed by a biochemical assay. Uses antibody capture for improved assay specificity\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Cell culture extracts, Tissue,\u003cbr\u003e\nReacts with:Mouse, Rat,\u003cbr\u003e\nAssay type:Enzyme activity (quantitative),\u003cbr\u003e\nAssay time:6h,\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nComplex IV Rodent Enzyme Activity Microplate Assay Kit (ab109911) is used to determine the activity of cytochrome c oxidase in a mouse sample with speed and simplicity.\u003cbr\u003e\nThe COX enzyme is immunocaptured within the wells of the microplate and activity is determined colorimetrically by following the oxidation of reduced cytochrome c by the absorbance change at 550 nm.\u003cbr\u003e\nIncluded in this kit for performance of the activity assay are buffer, detergent, substrate, and 96-well microplate with monoclonal antibody pre-bound to the wells of the plate, allowing for a stream-lined assay.\u003cbr\u003e\nComplex IV assay protocol summary\u003cbr\u003e\n- add samples to wells to capture enzyme and incubate for 3 hrs\u003cbr\u003e\n- wash wells\u003cbr\u003e\n- add reaction mix\u003cbr\u003e\n- analyze with microplate reader in kinetic mode for 120 min\u003cbr\u003e\nBuffer, detergent, and microplate should be stored at 4°C, Reagent C (reduced cytochrome c) should be stored at -80°C.\u003cbr\u003e\nRange of complex IV \/ cytochrome c oxidase assay kits\u003cbr\u003e\nImmunocapture with biochemical assay (plate-based) ab109911 (this kit) and\u003cbr\u003e\nab109909\u003cbr\u003e\nImmunocapture with biochemical assay (dipstick)\u003cbr\u003e\nab109878\u003cbr\u003e\nab109876\u003cbr\u003e\nImmunocapture with biochemical assay and ELISA\u003cbr\u003e\nab109910\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Dry Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nComplex IV also known as cytochrome c oxidase is an important component of the electron transport chain in mitochondria. This enzyme complex has significant mass approximately 204 kDa and operates primarily in the inner mitochondrial membrane. It catalyzes the transfer of electrons from cytochrome c to oxygen facilitating ATP generation. Complex IV is ubiquitously expressed in all tissues with higher expression in organs with high energy demands like heart and skeletal muscle. This enzyme is composed of multiple subunits encoded by both mitochondrial and nuclear DNA.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nCytochrome c oxidase plays a critical role in cellular respiration. It forms part of the larger enzyme complex which also includes Complex I II and III. These complexes work together to create a proton gradient across the inner mitochondrial membrane essential for ATP synthesis via oxidative phosphorylation. This enzyme's activity is measured through assays like the cytochrome c oxidase assay which evaluates its function in various tissues. These assays help researchers understand how effectively electrons are being transferred and protons are driven across the membrane.\u003cbr\u003e\nPathways\u003cbr\u003e\nComplex IV is integral to the oxidative phosphorylation pathway and the broader mitochondrial respiratory chain. It interacts closely with cytochrome c a small heme protein that shuttles electrons between Complex III (cytochrome c reductase) and Complex IV. Through these interactions the proton gradient is established enabling ATP synthase to convert chemical energy into usable cell energy. This process significantly impacts cellular metabolism and energy production influencing how efficiently cells function.\u003cbr\u003e\nComplex IV dysfunction is associated with mitochondrial diseases and certain neurodegenerative disorders. For instance defects in cytochrome c oxidase lead to conditions like Leigh syndrome a severe neurological disorder. Also studies show that disruptions in the electron transport chain involving Complex IV relate closely to Alzheimer's disease. Alterations in proteins like cytochrome c which work in tandem with Complex IV can exacerbate these conditions highlighting the importance of this target in understanding and potentially treating these diseases.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843538899113,"sku":"ab109911","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab109911","provider":"Iright","version":"1.0","type":"link"}