{"product_id":"abcam-ab110333","title":"Abcam, ab110333, Anti-Pyruvate dehydrogenase E2\/E3bp antibody [13G2AE2BH5]","description":"\u003cp\u003eSize: 100µg\u003cbr\u003e\nAnti-Pyruvate dehydrogenase E2\/E3bp antibody [13G2AE2BH5] (ab110333) is a mouse monoclonal antibody detecting PDHX in  Western Blot, Flow Cytometry, IHC-P, ICC\/IF . Suitable for  Cow, Human, Mouse, Rat . - KO validated for confirmed specificity - Over 40 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Mouse,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:13G2AE2BH5,\u003cbr\u003e\nIsotype:IgG2a,\u003cbr\u003e\nLight chain type:kappa,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Mouse, Rat, Cow, Human,\u003cbr\u003e\nApplications:Flow Cyt, WB, ICC\/IF, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWhat is this antibody validated in?\u003cbr\u003e\nAnti-Pyruvate dehydrogenase E2\/E3bp antibody [13G2AE2BH5] (ab110333) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry\/immunofluorescence (ICC\/IF) in Cow, Human, Mouse, Rat samples.\u003cbr\u003e\nWhat is the molecular weight of PDHX?\u003cbr\u003e\nAnti-Pyruvate dehydrogenase E2\/E3bp [13G2AE2BH5] (ab110333) specifically detects a band for PDHX (UniProt: O00330) at a molecular weight of 54 , 69kDa.\u003cbr\u003e\nTrusted by the scientific community\u003cbr\u003e\nAnti-Pyruvate dehydrogenase E2\/E3bp [13G2AE2BH5] (ab110333) was first used in a scientific publication in 2011 and has been cited over 40 times in peer-reviewed journals.\u003cbr\u003e\nReviewed by scientists\u003cbr\u003e\nAnti-Pyruvate dehydrogenase E2\/E3bp [13G2AE2BH5] (ab110333) has over 5 independent reviews from customers.\u003cbr\u003e\nSpecificity confirmed\u003cbr\u003e\nThe specificity of Anti-Pyruvate dehydrogenase E2\/E3bp antibody [13G2AE2BH5] (ab110333) has been confirmed by Western blot testing in DLAT Knockout HAP1 cells.\u003cbr\u003e\nWant a custom formulation?\u003cbr\u003e\nThis antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purity-IgG fraction, Purification notes-ab110333 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation., Storage buffer-pH: 7.5Preservative: 0.02% Sodium azideConstituents: HEPES buffered saline, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nPDHX also known as pyruvate dehydrogenase protein X is a part of the pyruvate dehydrogenase complex. It has a molecular mass of about 54 kDa. PDHX is expressed mostly in tissues with high energy demands such as the heart and skeletal muscle. The protein has a critical role in linking glycolysis and the Krebs cycle by transferring the lipoamide group in the pyruvate dehydrogenase complex.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nPDHX functions as a component of the multi-enzyme pyruvate dehydrogenase complex (PDC). This complex is essential for the oxidative decarboxylation of pyruvate to acetyl-CoA within the mitochondria. PDHX coordinates with other enzymes such as E1 E2 and E3 carrying out its function by acting as an anchor to stabilize the PDC enhancing the efficiency of the catalytic process.\u003cbr\u003e\nPathways\u003cbr\u003e\nPDHX participates in important metabolic pathways like glycolysis and the Krebs cycle. It relates closely with proteins such as E1 (pyruvate dehydrogenase) and E2 (dihydrolipoamide acetyltransferase) due to its role in facilitating the transition of energy substrates into the cycle. Its activity helps in the regulation of energy production within cells and impacts processes that depend on efficient energy conversion.\u003cbr\u003e\nMutations or deficiencies in PDHX have connections with pyruvate dehydrogenase deficiency and metabolic disorders like Leigh syndrome. These conditions affect energy metabolism and can lead to severe neurological problems. The malfunction of PDHX impacts the pyruvate dehydrogenase complex as a whole leading to an increase of pyruvate levels and a decrease in ATP production linking it also with other involved proteins like E1 and E2.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46844275490985,"sku":"ab110333","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab110333","provider":"Iright","version":"1.0","type":"link"}