{"product_id":"abcam-ab112128","title":"Abcam, ab112128, Fluo-8 Calcium Assay Kit - Medium Removal","description":"\u003cp\u003eSize: 1 x 96Tests \/ 10 x 96Tests \/ 100 x 96Tests\u003cbr\u003e\nFluo-8 Calcium Assay Kit - Medium Removal (ab112128) is a fluorescence-based assay for detecting intracellular calcium mobilization.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Fluorescent,\u003cbr\u003e\nSample types:Suspension cells, Adherent cells,\u003cbr\u003e\nAssay type:Cell-based,\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAbcam's Fluo-8 Calcium Assay Kit - Medium Removal (ab112128) is a fluorescence-based assay for detecting intracellular calcium mobilization. Cells expressing a GPCR of interest that signals through calcium are pre-loaded with Fluo-8 which can cross the cell membrane. Once inside the cell, the lipophilic blocking groups of Fluo-8 are cleaved by an esterase, resulting in a negatively charged fluorescent dye that stays inside the cell. Its fluorescence is greatly enhanced upon binding to calcium. When cells are stimulated with agonists, the receptor signals the release of intracellular calcium, which significantly increases the fluorescence of Fluo-8. The characteristics of its long wavelength, high sensitivity, and \u0026gt;100 times fluorescence enhancement make Fluo-8 the brightest green calcium indicator available in the marker, and it is an ideal tool for the measurement of cellular calcium through HTS screening.\u003cbr\u003e\nab112128 provides an optimized assay method for monitoring the G-protein-coupled receptors and calcium channels using HTS instrumentation. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format.\u003cbr\u003e\nFluo-8 products available\u003cbr\u003e\nFluo-8 Calcium Flux Assay Kits are available in No Wash and Medium Removal variants:\u003cbr\u003e\n- Calcium Flux Assay Kit (Fluo-8, No Wash)\u003cbr\u003e\nab112129\u003cbr\u003e\nis generally preferred as the workflow is simpler. It is also recommended for cell lines that are only weakly adherent and risk losing adherence when washing.\u003cbr\u003e\n- Medium removal kit Fluo-8 Calcium Flux Assay Kit ab112128 is recommended for the testing of compounds, receptors and calcium channels that are sensitive to the presence of cell culture media or serum. Alternatively, for cells that can tolerate it, consider using a low serum % in culture media and use No Wash Fluo-8 kit\u003cbr\u003e\nab112129\u003cbr\u003e\nFluo-8 is also available as a standalone biochemical\u003cbr\u003e\nab142773\u003cbr\u003e\nThis product is intended to be used for monitoring calcium fluctuations in vivo in live cells using the following HTS imaging plate readers: FLIPR™, FDSS, BMG NOVOstar™, FLexStation, ViewLux, IN Cell Analyzer or Arrayscan.\u003cbr\u003e\nIf you would like to quantify calcium concentration\u003cbr\u003e\nin vitro\u003cbr\u003e\nusing cell extracts, we recommend using Calcium Detection Kit (Colorimetric) (\u003cbr\u003e\nab102505\u003cbr\u003e\n) or Calcium Quantification Assay Kit (\u003cbr\u003e\nab112115\u003cbr\u003e\n), as they provide stock standards for comparison.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855623508137,"sku":"ab112128","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab112128","provider":"Iright","version":"1.0","type":"link"}