{"product_id":"abcam-ab113688","title":"Abcam, ab113688, Anti-PHO8 antibody [1D3A10]","description":"\u003cp\u003eSize: 250µg\u003cbr\u003e\nMouse Monoclonal PPB antibody. Suitable for WB, ICC\/IF and reacts with Saccharomyces cerevisiae samples. Cited in 18 publications.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Mouse,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:1D3A10,\u003cbr\u003e\nIsotype:IgG1,\u003cbr\u003e\nLight chain type:kappa,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Saccharomyces cerevisiae,\u003cbr\u003e\nApplications:WB, ICC\/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWant a custom formulation?\u003cbr\u003e\nThis antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Proprietary technique, Purification notes-Near homogeneity (Purity \u0026gt;95% by SDS-PAGE). The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by chemical fractionation., Storage buffer-pH: 7.5Preservative: 0.02% Sodium azideConstituents: HEPES buffered saline, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\n'PHO8' also known as yeast alkaline phosphatase is an enzyme that plays an important role in phosphate metabolism in Saccharomyces cerevisiae. PHO8 is a homodimer with a mass of approximately 70 kDa per subunit. Its expression predominantly occurs in the vacuole where it functions optimally in an acidic environment. The enzyme facilitates the dephosphorylation processes by removing phosphate groups from various substrates making it essential for phosphate salvage.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nPHO8 is critical for cellular phosphate homeostasis. It operates as part of a larger complex situated in the vacuole membrane. The enzyme assists in breaking down phosphomonoesters into inorganic phosphate which can then be reused by the cell. This activity is particularly important under conditions of nutrient limitation where maintaining phosphate balance becomes important. PHO8's regulation is tightly linked to environmental phosphate levels ensuring that phosphate is efficiently managed within the cell.\u003cbr\u003e\nPathways\u003cbr\u003e\nPHO8 is integral to the phosphate regulatory (PHO) signaling pathway. In this network the enzyme cooperates with proteins like PHO4 and PHO2 to regulate phosphate uptake and storage. The coordination between these proteins enables the cell to adjust phosphate metabolism according to availability. Additionally PHO8 also interacts with related pathways involving the vacuole's function in storage and turnover of phosphate compounds further emphasizing its role in cellular nutrient management.\u003cbr\u003e\nPHO8's influence extends to conditions impacting phosphate metabolism. Although largely studied in yeast insights from PHO8 functionality contribute to understanding human disorders related to phosphate dysregulation such as hypophosphatasia. Interestingly studies suggest that homologous human alkaline phosphatases share a similar regulatory mechanism influenced by the protein PHO85 in yeast shedding light on potential parallels in disease presentation and treatment strategies.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46854737821865,"sku":"ab113688","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab113688","provider":"Iright","version":"1.0","type":"link"}