{"product_id":"abcam-ab113850","title":"Abcam, ab113850, JC-1 - Mitochondrial Membrane Potential Assay Kit","description":"\u003cp\u003eSize: 100Test\u003cbr\u003e\nJC-1 - Mitochondrial Membrane Potential Assay Kit ab113850 is used for the measurement of mitochondrial membrane potential by fluorescence plate reader. In the assay, the cationic dye JC-1 accumulates in mitochondria in a membrane potential-dependent manner, forming red-fluorescent aggregates in polarized mitochondria and remaining as green-fluorescent monomers in depolarized mitochondria. - Includes FCCP as a control - Cited in \u0026gt; 140 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Fluorescent,\u003cbr\u003e\nSample types:Suspension cells, Adherent cells,\u003cbr\u003e\nAssay time:1h,\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nJC-1 - mitochondrial membrane potential assay kit uses tetraethylbenzimidazolylcarbocyanine iodide (JC-1), a cationic dye that accumulates in energized mitochondria to measure the mitochondrial membrane potential. At low concentrations (due to low membrane potential) JC-1 is predominantly a monomer that yields green fluorescence with emission of 530±15 nm. At high concentrations (due to high membrane potential) the dye aggregates yielding a red to orange colored emission (590±17.5 nm). Therefore, a decrease in the aggregate fluorescent count is indicative of depolarization whereas an increase is indicative of hyperpolarization. The accompanying FCCP (carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone) is an ionophore uncoupler of oxidative phosphorylation. Treating cells with FCCP eliminates mitochondrial membrane potential and JC1 staining. JC1 is suitable for the labeling of mitochondria in live cells and it is not compatible with fixation.\u003cbr\u003e\nJC-1 - Mitochondrial Membrane Potential Assay Kit protocol summary\u003cbr\u003e\n- Wash cells in dilution buffer or PBS\u003cbr\u003e\n- Add JC solution\u003cbr\u003e\n- Incubate for 30 min at 37°C for suspension cells, or 10 min for adherent cells\u003cbr\u003e\n- Wash cells with dilution buffer\u003cbr\u003e\n- Treat cells as desired for experimental plan\u003cbr\u003e\n- Analyze on a fluorescent microplate reader\u003cbr\u003e\nThe aggregate dye can be excited at 535 nm, the monomer and aggregate together at 475 nm.\u003cbr\u003e\nJC-1 - Mitochondrial Membrane Potential Assay Kit has been used in a variety of sample type including:\u003cbr\u003e\nHuman fibroblasts and kidney cells\u003cbr\u003e\nHuman astrocytes\u003cbr\u003e\nHuman primary gingival fibroblasts\u003cbr\u003e\nReferences:\u003cbr\u003e\n1- Ziegler D et al. 2024\u003cbr\u003e\n2-Mekhaeil M et al. 2023\u003cbr\u003e\n3-Szymanska E et al. 2023\u003cbr\u003e\nRelated and recommended products\u003cbr\u003e\nSee other alternative kits to quantify Mitochondrial Membrane Potential:\u003cbr\u003e\n- JC-10 Mitochondrial Membrane Potential Assay Kit (Microplate)\u003cbr\u003e\nab112134\u003cbr\u003e\n- JC-10 Mitochondrial Membrane Potential Assay Kit (Flow Cytometry)\u003cbr\u003e\nab112133\u003cbr\u003e\n- TMRE-Mitochondrial Membrane Potential Assay Kit\u003cbr\u003e\nab113852\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe mitochondrial membrane potential also known as ΔΨm is the electrical potential difference across the inner mitochondrial membrane. This potential results from the electrochemical gradient produced by the proton pumps during electron transport chain activity. The mechanical function of the mitochondrial membrane potential is important to ATP production through oxidative phosphorylation. Mitochondrial membranes are widely expressed in almost all eukaryotic cells and are an essential component of cellular metabolism. The inner membrane is structured to facilitate its function housing integral proteins that are key to maintaining the potential.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe mitochondrial membrane potential drives ATP synthesis by powering ATP synthase an enzyme complex embedded in the mitochondrial membrane. This potential also plays a vital role in other processes such as calcium homeostasis and regulation of mitochondrial biogenesis. The mitochondrial membrane itself forms part of the larger mitochondrial respiratory chain complex coordinating with components like complex I (NADH: ubiquinone oxidoreductase) and complex II (succinate dehydrogenase) to maintain cell energy needs and respond to metabolic demands.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe mitochondrial membrane potential is integral to cellular energy metabolism pathways such as the Krebs cycle and oxidative phosphorylation. Mitochondrial membrane potential modulation can affect signaling proteins like cytochrome c which is instrumental in apoptosis. Apoptotic signaling pathways involving proteins such as Bax and Bcl-2 influence the mitochondrial membrane potential and regulate cell survival or death in response to cellular stress or damage.\u003cbr\u003e\nChanges in the mitochondrial membrane potential relate significantly to conditions like neurodegenerative diseases and cancer. In neurodegenerative diseases such as Parkinson’s and Alzheimer's dysregulation of mitochondrial membrane potential can lead to impaired energy production and increased oxidative stress. Cancer cells often exhibit altered mitochondrial membrane potential affecting processes like apoptosis and enabling survival in adverse conditions. These alterations in potential impact proteins such as p53 which play critical roles in cancer progression and neurodegenerative disease pathology.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843541651625,"sku":"ab113850","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab113850","provider":"Iright","version":"1.0","type":"link"}