{"product_id":"abcam-ab116027","title":"Abcam, ab116027, Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa)","description":"\u003cp\u003eSize: 500µL\u003cbr\u003e\n10-180 kDa prestained protein ladder (ab116027) is a ready- to-use, three-color protein standard with 10 pre-stained proteins ranging from molecular weights 10 to 180 kDa. - Suitable for western blot (WB) and SDS-PAGE - Suitable for Tris Glycine (4-12%) SDS buffer, Bis-Tris (4-12%) MOPS buffer and Bis-Tris (4-12%) MES buffer - Part of our Lab essential range western blot workflow\u003cbr\u003e\nKey facts\u003cbr\u003e\nApplications:WB, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nStorage buffer:pH: 7.5Constituents: 15% Glycerol (glycerin, glycerine), 3% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 2% Sodium lauryl sulfate, 1% Tris phosphate, 0.02% Urea\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nPrestained Protein Ladder ab116027 is a three-color protein standard with 10 pre-stained proteins covering a wide range molecular weights for 10 to 180 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer).\u003cbr\u003e\nThis product was previously called Prism Ultra Protein Ladder (10 - 180 kDa).\u003cbr\u003e\nThis prestained protein ladder is designed for monitoring protein separated during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximate sizing of proteins. The ladder is supplied in gel loading buffer and is ready to use.\u003cbr\u003e\nDo not heat, dilute, add reducing agent before loading.\u003cbr\u003e\nKey product features:\u003cbr\u003e\nBroad range:\u003cbr\u003e\n10-180 kDa\u003cbr\u003e\nReady-to-use:\u003cbr\u003e\nSupplied in a loading buffer for direct loading on gels.\u003cbr\u003e\nEasy to identify:\u003cbr\u003e\nIncludes green ~25 kDa and red ~75kDa reference bands.\u003cbr\u003e\nSharp bands\u003cbr\u003e\nRecommended loading:\u003cbr\u003e\n~2 - 3 μl for western blots\u003cbr\u003e\nReview other protein ladders in the\u003cbr\u003e\nunstained and prestained protein ladder guide\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847571722409,"sku":"ab116027","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab116027","provider":"Iright","version":"1.0","type":"link"}