{"product_id":"abcam-ab117130","title":"Abcam, ab117130, Hydroxymethylated DNA Quantification Kit (Colorimetric)","description":"\u003cp\u003eSize: 48Test \/ 96Test\u003cbr\u003e\nDNA methylation is a covalent modification of the cytosine ring at the 5' position, resulting in 5-methylcytosine (5-mC).\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Saliva, Urine, Plasma, Tissue, Suspension cells, Serum, Adherent cells,\u003cbr\u003e\nAssay type:Quantitative,\u003cbr\u003e\nSensitivity:\u0026gt;= 40 pg\/well,\u003cbr\u003e\nAssay time:3h 45m,\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nDNA methylation is a covalent modification of the cytosine ring at the 5' position, resulting in 5-methylcytosine (5-mC). In somatic cells, 5-mC is found almost exclusively in the context of paired symmetrical methylation of the dinucleotide CpG. The biological important of 5-mC as a major epigenetic modification in phenotype and gene expression has been recognized widely. Quite recently, a novel modified nucleotide, 5-hydroxymethylcytosine (5-hmC), has been detected to be abundant in mouse brain and embryonic stem cells. It is a hydroxylated and methylated form of cytosine. Although it is still uncertain its specific role in epigenetics, it is believed it plays a role in DNA demethylation, chromatin remodeling and gene expression regulation.\u003cbr\u003e\nAbcam's Hydroxymethylated DNA Quantification Kit (Colorimetric) (ab117130) is a complete set of optimized buffers and reagents to quantify global DNA hydroxymethylation by specifically measuring levels of 5-hydroxymethylcytosine (5-hmC) without cross-reactivity to methylcytosine and unmethylated cytosine.\u003cbr\u003e\nIt can be paired to use with Methylated DNA Quantification Kit (Colorimetric) (\u003cbr\u003e\nab117128\u003cbr\u003e\n), which specifically quantifies 5-mC, for simultaneously quantification of both methylated and hydroxymethylated DNA.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843646247081,"sku":"ab117130","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab117130","provider":"Iright","version":"1.0","type":"link"}