{"product_id":"abcam-ab119211","title":"Abcam, ab119211, InstantBlue® Coomassie Protein Stain (ISB1L)","description":"\u003cp\u003eSize: 1000mL\u003cbr\u003e\nInstantBlue® Coomassie protein stain (ISB1L) is a ready-to-use, non-toxic, ultra-fast Coomassie blue dye to stain protein gels delivering clear results in 15 minutes. Uses a single-step protocol eliminating the need to wash, fix or destain. - Coomassie InstantBlue® protein stains proven performance: cited in over 595 publications - Stains at least 40 mini gels per bottle (25 mL\/mini gel) - No gel shrinkage or protein methylation\/acetylation - High sensitivity - detects protein levels as low as 5 ng per band - Compatible with Mass Spectrometry\u003cbr\u003e\nKey facts\u003cbr\u003e\nForm:LiquidSee storage information\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nInstantBlue\u003cbr\u003e\n(ISB1L)  is a ready to use, ultra-fast and sensitive Coomassie Blue protein stain for polyacrylamide gels. Protein gels can be stained in 15 minutes in a one-step protocol eliminating the need to fix, wash or destain.  It is formulated for safe use and easy disposal and comes in a convenient premixed one liter bottle.\u003cbr\u003e\nWhy InstantBlue\u003cbr\u003e\nCoomassie Protein Stain?\u003cbr\u003e\nWhen compared to homemade and some premade Coomassie Blue solutions, InstantBlue\u003cbr\u003e\ndoes not require additional steps to wash, fix, microwave and destain. Coomassie InstantBlue\u003cbr\u003e\nuses a simple one-step protocol saving you time.\u003cbr\u003e\nThe unique patented mechanism for rapid Coomassie blue staining of protein gels begins in moments, and results are achieved within 15 minutes. Some premade and traditional homemade Coomassie R-250 protein stains can take three hours or more to fully stain gels, and then typically require destaining overnight.\u003cbr\u003e\nUnparalleled sensitivity allows protein levels as low as 5ng per band to be detected (BSA) when staining overnight. Coomassie InstantBlue\u003cbr\u003e\nprovides specific staining, which gives a clear background, improving the signal \/ noise ratio of the stain. Despite the staining power, gels can be left in Coomassie InstantBlue\u003cbr\u003e\nfor weeks and still come out with a crystal clear background, all without the need for destaining or protein stain remover.\u003cbr\u003e\nThe nontoxic formula is sink disposable and its methanol free composition will not shrink gels. Other stains require solvent disposal procedures and can require microwaving, which produces dangerous fumes. The effective staining and safe composition of InstantBlue means that there's no need for microwaving, solvent disposal, or fume hoods.\u003cbr\u003e\nCoomassie InstantBlue\u003cbr\u003e\nwill stain at least 40 mini gels per bottle, boasting economical staining with only 25ml per mini gel. As a result, this allows you to use less protein staining solution when compared to 50–100ml of stain per gel for homemade solutions and some premixed solutions.\u003cbr\u003e\nFully compatible with mass spectrometry, gels can be destained using standard procedures. The protein staining solution's acetic acid free formulation means it will not methylate or acetylate protein during the protein staining protocol.\u003cbr\u003e\nKey Benefits\u003cbr\u003e\nUltra fast staining\u003cbr\u003e\n– Results in 15 minutes or less\u003cbr\u003e\nOne step procedure\u003cbr\u003e\n– No washing, fixing, microwaving or destaining\u003cbr\u003e\nHigh sensitivity\u003cbr\u003e\n– 5ng bands detectable\u003cbr\u003e\nClear background\u003cbr\u003e\n– High signal \/ noise ratio\u003cbr\u003e\nFlexible\u003cbr\u003e\n– No overstaining\u003cbr\u003e\nReliable –\u003cbr\u003e\nStringent batch to batch consistency\u003cbr\u003e\nEfficient\u003cbr\u003e\n– Only 25ml per gel\u003cbr\u003e\nSafe composition\u003cbr\u003e\n– Nontoxic; no fume hood or solvent disposal required\u003cbr\u003e\nMethanol free\u003cbr\u003e\n– No gel shrinkage or protein methylation\u003cbr\u003e\nAcetic acid free\u003cbr\u003e\n– No protein acetylation\u003cbr\u003e\nMass spectrometry compatible\u003cbr\u003e\n– Destainable\u003cbr\u003e\nCoomassie Blue staining protocol when using InstantBlue\u003cbr\u003e\n(ISB1L)\u003cbr\u003e\nBEFORE USE: Mix the Coomassie InstantBlue\u003cbr\u003e\nsolution immediately before using by gently inverting the bottle a few times (do not shake the bottle to mix the solution).\u003cbr\u003e\n- Multiple washes prior to staining with Coomassie InstantBlue\u003cbr\u003e\nare NOT required or recommended.\u003cbr\u003e\n- An alcohol\/acetic acid fixing step prior to staining with Coomassie InstantBlue\u003cbr\u003e\nis NOT required or recommended.\u003cbr\u003e\n- A destaining step post staining is NOT required or recommended with Coomassie InstantBlue\u003cbr\u003e\nStandard Coomassie Blue staining Protocol:\u003cbr\u003e\n1. After electrophoresis remove the gel from the tank and transfer directly into the Coomassie InstantBlue\u003cbr\u003e\nstaining solution. Be sure that the gel moves freely in stain to facilitate diffusion. Typically, ~20 mL is needed to cover the gel.\u003cbr\u003e\n2. Coloured protein bands will start to develop immediately, and a suitable intensity is typically achieved after 15 minutes incubation at room temperature with gentle shaking.\u003cbr\u003e\n3. Photograph your gel when the required intensity has been achieved. Gels can be kept in staining solution but ensure that the gel remains covered with liquid. Alternatively, the gel can be stored in ultrapure water after staining for 1 hour in Commassie InstantBlue\u003cbr\u003e\nThis product is manufactured by Expedeon, an Abcam company. Expedeon product code ISB1L was previously called InstantBlue Protein Stain 1 L and is the same as the 1000 ml size of this product.\u003cbr\u003e\nManufactured under patent numbers : US 12\/226,797 ; SG 201103010-3 ; SG 200807953-5 ; JP 2009-507182 ; IN 5722\/CHENP\/2008 ; GB 0608377.8 ; EP 10187154.9; EP 07733647.7 ; CN 200780021434.8 ; CA 2,649,835 ; AU 2007245443\u003cbr\u003e\nINSTANTBLUE is a registered trademark of Abcam in the US and an unregistered trademark elsewhere\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847570444457,"sku":"ab119211","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab119211","provider":"Iright","version":"1.0","type":"link"}