{"product_id":"abcam-ab119531","title":"Abcam, ab119531, Mouse IL-12 p70 ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nMouse IL-12 p70 ELISA Kit is a sandwich ELISA designed to quantify Mouse IL-12 p70 with a sensitivity of 10 pg\/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 15.6 - 1000 pg\/mL\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Cell culture supernatant, Serum,\u003cbr\u003e\nReacts with:Mouse,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 10 pg\/mL,\u003cbr\u003e\nRange:15.6 - 1000 pg\/mL,\u003cbr\u003e\nAssay Platform:Microplate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nMouse IL-12 p70 ELISA Kit ab119531 is a sandwich ELISA to measure Mouse IL-12 p70 in serum, cell culture supernatant with a sensitivity of 10 pg\/ml.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nIL-12 p70 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and along with a Biotin-conjugated anti-mouse IL-12 p70 detection antibody and the microplate is then incubated at room temperature. Following washing with wash buffer a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and then unbound conjugates are washed away using wash buffer. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-12 p70 captured on the plate.\u003cbr\u003e\nAssay Specificity\u003cbr\u003e\nOur ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details\u003cbr\u003e\nMouse IL-12 p70 ELISA Kit ab119531 protocol summary\u003cbr\u003e\n1. Add standard or sample to appropriate wells. Incubate the plate\u003cbr\u003e\n2. Wash and add Biotinylated mouse anti-TGF beta 1 monoclonal antibody to appropriate wells. Incubate the plate.\u003cbr\u003e\n3. Wash and add prepared Streptavidin-HRP Conjugate to appropriate wells. Incubate at room temperature\u003cbr\u003e\n4. Wash and add TMB Substrate to each well\u003cbr\u003e\n5. Add Stop Solution to each well. Read immediately\u003cbr\u003e\nThe standard is affinity purified.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843549024425,"sku":"ab119531","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab119531","provider":"Iright","version":"1.0","type":"link"}