{"product_id":"abcam-ab119542","title":"Abcam, ab119542, Human IL-21 ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman IL-21 ELISA Kit is a sandwich ELISA designed to quantify Human IL-21 with a sensitivity of 20 pg\/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 78 - 5000 pg\/mL\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Plasma, Cell culture supernatant, Serum,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 20 pg\/mL,\u003cbr\u003e\nRange:78 - 5000 pg\/mL,\u003cbr\u003e\nAssay Platform:Microplate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman IL-21 ELISA Kit ab119542 is a sandwich ELISA to measure Human IL-21 in serum, plasma, cell culture supernatant with a sensitivity of 20 pg\/ml.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nIL-21 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotin-conjugated IL-21 detection antibody then incubated at room temperature. Following washing, a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-21 captured on the plate.\u003cbr\u003e\nAssay Specificity\u003cbr\u003e\nOur ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details\u003cbr\u003e\nHuman IL-21 ELISA Kit ab119542 protocol summary\u003cbr\u003e\n1. Add standard or sample to appropriate wells. Incubate the plate\u003cbr\u003e\n2. Wash and add Biotinylated mouse anti-TGF beta 1 monoclonal antibody to appropriate wells. Incubate the plate.\u003cbr\u003e\n3. Wash and add prepared Streptavidin-HRP Conjugate to appropriate wells. Incubate at room temperature\u003cbr\u003e\n4. Wash and add TMB Substrate to each well\u003cbr\u003e\n5. Add Stop Solution to each well. Read immediately\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843631796393,"sku":"ab119542","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab119542","provider":"Iright","version":"1.0","type":"link"}