{"product_id":"abcam-ab119557","title":"Abcam, ab119557, Mouse TGF beta 1 ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nMouse TGF beta 1 ELISA Kit is a sandwich ELISA designed to quantify Mouse TGF beta 1 with a sensitivity of 7.8 pg\/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 31.3 - 2000 pg\/mL - Cited in over 60 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Plasma, Cell culture supernatant, Serum,\u003cbr\u003e\nReacts with:Mouse,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 7.8 pg\/mL,\u003cbr\u003e\nRange:31.3 - 2000 pg\/mL,\u003cbr\u003e\nAssay Platform:Microplate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nMouse TGF beta 1 ELISA Kit ab119557 is a sandwich ELISA to measure Mouse TGF beta 1 in serum, plasma, cell culture supernatant with a sensitivity of 7.8 pg\/ml.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nMouse TGF beta 1 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and then incubated at room temperature. After washing, a Biotin-conjugated anti-Mouse TGF beta 1 detection antibody is added then incubated at room temperature. Following washing Streptavidin-HRP conjugate is added to each well, incubated at room temperature then again washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after the addition of an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Mouse TGF beta 1 captured on the plate\u003cbr\u003e\nAssay Specificity\u003cbr\u003e\nOur ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details\u003cbr\u003e\nMouse TGF beta 1 ELISA Kit ab119557 protocol summary\u003cbr\u003e\n1. Add standard or sample to appropriate wells. Incubate the plate\u003cbr\u003e\n2. Wash and add Biotinylated mouse anti-TGF beta 1 monoclonal antibody to appropriate wells. Incubate the plate.\u003cbr\u003e\n3. Wash and add prepared Streptavidin-HRP Conjugate to appropriate wells. Incubate at room temperature\u003cbr\u003e\n4. Wash and add TMB Substrate to each well\u003cbr\u003e\n5. Add Stop Solution to each well. Read immediately\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843540013225,"sku":"ab119557","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab119557","provider":"Iright","version":"1.0","type":"link"}