{"product_id":"abcam-ab124728","title":"Abcam, ab124728, Anti-UBA3 antibody [MILR42872]","description":"\u003cp\u003eSize: 100µL \/ 1mL\u003cbr\u003e\nRabbit Recombinant Monoclonal UBA3 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 12 publications.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:MILR42872,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Mouse, Human,\u003cbr\u003e\nApplications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.05% Sodium azideConstituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Stable for 12 months at -20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe target UBA3 also known as ubiquitin-like modifier-activating enzyme 3 functions mechanically as an essential enzyme in the NEDD8-conjugation pathway. It weighs approximately 50 kDa. UBA3 expresses itself in various tissues but shows widespread expression across different cell types. It operates as a catalytic subunit of the E1 enzyme complex that initiates the activation and transfer of the NEDD8 protein. This process is important for the subsequent conjugation to target proteins.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe process involving UBA3 relates to protein modification primarily through neddylation. UBA3 couples with the protein NAE1 to form the NEDD8 activating enzyme complex. This enzyme complex activates NEDD8 a ubiquitin-like protein that modifies cullin-based E3 ligases influencing their function. By modifying these E3 ligases UBA3 controls various cellular processes including cell cycle progression and signal transduction ensuring proper cell function and adaptation to environmental cues.\u003cbr\u003e\nPathways\u003cbr\u003e\nUBA3 is integral to the NEDD8 conjugation pathway influencing cell cycle and transcriptional regulation. It interacts with proteins such as cullin 1 and cullin 3 within this pathway. By activating NEDD8 UBA3 facilitates cullin-RING ubiquitin ligase complexes which play significant roles in the ubiquitin-proteasome pathway. This interaction controls protein degradation a vital process for maintaining protein homeostasis and preventing cellular dysfunction.\u003cbr\u003e\nUBA3 involvement in neddylation links it to cancer and neurodegenerative diseases. Aberrant UBA3 activity relates to cancers as dysregulation of the NEDD8 pathway can lead to uncontrolled cell proliferation. In neurodegenerative disorders such as Alzheimer's disease altered neddylation can disrupt normal protein homeostasis contributing to neuron damage. UBA3's interaction with protein partners like cullins connects it to various pathological states highlighting its importance in disease development and progression.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46848552763561,"sku":"ab124728","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab124728","provider":"Iright","version":"1.0","type":"link"}