{"product_id":"abcam-ab125965","title":"Abcam, ab125965, Human Complement C6 ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman Complement C6 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Complement C6 in Human in Biofluids samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Cerebral Spinal Fluid, Saliva, Urine, Plasma, Milk, Serum,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 0.14 ng\/mL,\u003cbr\u003e\nRange:0.391 - 25 ng\/mL,\u003cbr\u003e\nAssay time:4h,\u003cbr\u003e\nAssay Platform:Microplate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAbcam's Complement C6 Human\u003cbr\u003e\nin vitro\u003cbr\u003e\nELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of C6 in Human plasma, serum, saliva, urine, milk, and cell culture supernatants.\u003cbr\u003e\nA Complement C6 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Complement C6 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Complement C6 captured in plate.\u003cbr\u003e\nThe entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nComplement component C6 often called simply C6 is an essential protein in the complement system. It is part of the terminal complement pathway which leads to the formation of the membrane attack complex (MAC). C6 has a molecular weight of about 93 kDa and is mainly produced in the liver. It circulates in plasma and interacts with other complement proteins to form MAC aiding in pathogen elimination and cell lysis.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe role of C6 is the progression and stabilization of the MAC complex which is part of the innate immune system's defense. C6 collaborates with other complement proteins such as C5b C7 C8 and C9. This cooperation helps penetrate the membrane of pathogenic cells leading to cell destruction. Detecting and quantifying C6 levels is possible using techniques like complement C6 ELISA and C6 graphs valuable for research into immune response mechanisms.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe complement component C6 is an important player in the terminal complement pathway and the larger complement activation pathway. Within these pathways C6 interacts closely with proteins like C5b and C9 to facilitate the assembly of the MAC. C6's involvement is critical for mounting an efficient immune response against invading pathogens making it an important focus for immunology studies involving the complement cascade.\u003cbr\u003e\nC6 deficiencies are linked to increased susceptibility to infections particularly by Neisseria species which cause diseases like meningitis. Individuals with C6-related complement deficiencies might show recurrent bacterial infections highlighting its role in immune defense. The protein's interplay with other complement components notably C5 and C7 can influence the disease states and inform potential therapeutic approaches aimed at boosting host defense mechanisms.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855610597545,"sku":"ab125965","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab125965","provider":"Iright","version":"1.0","type":"link"}