{"product_id":"abcam-ab133045","title":"Abcam, ab133045, Mouse IgG1 ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nMouse IgG1 ELISA Kit is a sandwich ELISA designed to quantify Mouse IgG1 with a sensitivity of 0.116 ng\/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 7.81 - 250 ng\/mL\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Cell culture supernatant, Serum,\u003cbr\u003e\nReacts with:Mouse,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 0.116 ng\/mL,\u003cbr\u003e\nRange:7.81 - 250 ng\/mL,\u003cbr\u003e\nAssay time:90m,\u003cbr\u003e\nAssay Platform:Microplate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nMouse IgG1 ELISA Kit ab133045 is a sandwich ELISA to measure Mouse IgG1 in serum, cell culture supernatant with a sensitivity of 0.116 ng\/ml.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nspecific antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and along with an HRP-conjugated IgG\u003cbr\u003e\ndetection antibody and the microplate is then incubated at room temperature. After the removal of unbound proteins by washing, TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a colored product that changes after adding acidic stop solution. The density of coloration is directly proportional to the IgG\u003cbr\u003e\namount of sample captured in plate.\u003cbr\u003e\nAssay Specificity\u003cbr\u003e\nOur ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details\u003cbr\u003e\nMouse IgG1 ELISA Kit ab133045 protocol summary\u003cbr\u003e\n1. Add standard or sample to each well used. Incubate at room temperature.\u003cbr\u003e\n2. Aspirate and wash each well.\u003cbr\u003e\n3. Add prepared HRP labeled secondary detector antibody. Incubate at room temperature\u003cbr\u003e\n4. Aspirate and wash each well.\u003cbr\u003e\n5. Add TMB Substrate Solution to each well.\u003cbr\u003e\n6. Immediately begin recording the color development\u003cbr\u003e\nIgG is divided into four subclasses; IgG1, IgG2, IgG3, and IgG4. IgG\u003cbr\u003e\nis the most abundant immunoglobulin found in the blood. It is a glycoprotein which consists of two identical heavy chains (50 kDa each) and two identical light chains (25 kDa each), to give a combined mass of approximately 150 kDa. The chains are held in place by covalent disulfide bonds. Each light chain contains two immunoglobulin (Ig) domains, while the heavy chains contain four Ig domains each. In the middle of each heavy chain is a relative varying portion called the \"hinge region\" which is unique to each IgG. This region allows for molecular flexibility and sets IgG\u003cbr\u003e\napart from its IgG counterparts. IgG1 properties and functions include neutralization, opsonization, activation of the complement system, diffusion into extravascular sites and crossing the placenta.\u003cbr\u003e\nCross Reactivity\u003cbr\u003e\nCompound\u003cbr\u003e\nCross Reactivity\u003cbr\u003e\nRat IgG1\u003cbr\u003e\nMouse IgG2b\u003cbr\u003e\nHuman IgG1\u003cbr\u003e\n\u0026lt;0.01%\u003cbr\u003e\nMouse IgG2a\u003cbr\u003e\n\u0026lt;0.01%\u003cbr\u003e\nMouse IgG3\u003cbr\u003e\n\u0026lt;0.01%\u003cbr\u003e\nMouse IgM\u003cbr\u003e\n\u0026lt;0.01%\u003cbr\u003e\nRecommended dilution of serum is 1:20,000\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843567341737,"sku":"ab133045","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab133045","provider":"Iright","version":"1.0","type":"link"}