{"product_id":"abcam-ab133046","title":"Abcam, ab133046, Mouse IgG2a ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nMouse IgG2a ELISA Kit is a sandwich ELISA designed to quantify Mouse IgG2a with a sensitivity of 47.3 pg\/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 7.81 - 250 ng\/mL\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Serum, Tissue Culture Media,\u003cbr\u003e\nReacts with:Mouse,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 47.3 pg\/mL,\u003cbr\u003e\nRange:7.81 - 250 ng\/mL,\u003cbr\u003e\nAssay Platform:Microplate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nMouse IgG2a ELISA Kit ab133046 is a sandwich ELISA to measure Mouse IgG2a in serum, tissue culture media with a sensitivity of 47.3 pg\/ml.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nspecific antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and along with an HRP-conjugated IgG\u003cbr\u003e\ndetection antibody and the microplate is then incubated at room temperature. After the removal of unbound proteins by washing, TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a colored product that changes after adding acidic stop solution. The density of coloration is directly proportional to the IgG\u003cbr\u003e\namount of sample captured in plate.\u003cbr\u003e\nAssay Specificity\u003cbr\u003e\nOur ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details\u003cbr\u003e\nMouse IgG2a ELISA Kit ab133046 protocol summary\u003cbr\u003e\n1. Add standard or sample to each well used. Incubate at room temperature.\u003cbr\u003e\n2. Aspirate and wash each well.\u003cbr\u003e\n3. Add prepared HRP labeled secondary detector antibody. Incubate at room temperature\u003cbr\u003e\n4. Aspirate and wash each well.\u003cbr\u003e\n5. Add TMB Substrate Solution to each well.\u003cbr\u003e\n6. Immediately begin recording the color development\u003cbr\u003e\nIgG2 is divided into two subclasses; IgG2a and IgG2b. It is a glycoprotein which consists of two identical heavy chains (50 kDa each) and two identical light chains (25 kDa each), to give a combined mass of approximately 150 kDa. The chains are held in place by covalent disulfide bonds. Each light chain contains two immunoglobulin (Ig) domains, while the heavy chains contain four Ig domains each. In the middle of each heavy chain is a relative varying portion called the \"hinge region\" which is unique to each IgG. This region allows for molecular flexibility and sets IgG2a apart from its IgG counterparts. Properties of IgG2a include neutralization of toxins and diffusion in the extracellular space.\u003cbr\u003e\nCross Reactivity\u003cbr\u003e\n% Cross Reactivity\u003cbr\u003e\nmouse IgG2a\u003cbr\u003e\nmouse IgG3\u003cbr\u003e\nmouse IgM\u003cbr\u003e\nmouse IgG1\u003cbr\u003e\nmouse IgG2b\u003cbr\u003e\nrat IgG2a\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843575730345,"sku":"ab133046","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab133046","provider":"Iright","version":"1.0","type":"link"}