{"product_id":"abcam-ab133050","title":"Abcam, ab133050, Oxytocin ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nOxytocin ELISA Kit is a competitive ELISA designed to quantify Oxytocin with a sensitivity of 15 pg\/mL - Colorimetric competitive ELISA - 450 nm readout - works on any plate reader - Validated on a number of sample types including cerebrospinal fluid (CSF) - Wide dynamic range - quantifies 15.6 - 1000 pg\/ml\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Cerebral Spinal Fluid, Saliva, Urine, Plasma, Cell culture supernatant, Milk, Serum, Tissue Culture Media,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Competitive,\u003cbr\u003e\nSensitivity:= 15 pg\/mL,\u003cbr\u003e\nRange:15.6 - 1000 pg\/mL,\u003cbr\u003e\nAssay time:25h,\u003cbr\u003e\nAssay Platform:Microplate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAbcam's Oxytocin\u003cbr\u003e\nin vitro\u003cbr\u003e\ncompetitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Oxytocin in Tissue Culture media, Human Serum, Saliva, Cerebrospinal Fluid and Urine.\u003cbr\u003e\nA goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-Oxytocin antigen and a polyclonal rabbit antibody specific to Oxytocin. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of Oxytocin captured in the plate.\u003cbr\u003e\nOxytocin is a neurohypophysial peptide which is produced in the paraventricular nuclei of the hypothalamus and stored in the posterior pituitary. The molecule consists of nine amino acids linked with a [1-6] disulfide bond and a semi-flexible carboxyamidated tail. A hormone once thought to be limited to female smooth muscle reproductive physiology, more current findings have determined that oxytocin also functions as a neurotransmitter, may be involved in neuropsychiatric disorders, social\/sexual behavior and is important in male reproductive physiology. Oxytocin and the related neurohypophysial peptide, Arg-Vasopressin, maintain renal water and sodium balance.\u003cbr\u003e\nHighly conserved across species boundaries, oxytocin-like neurohypophysial peptides are substituted primarily at residues 4 and\/or 8. In the Oxytocin-like peptide, mesotocin, a common peptide found in some fishes, reptiles, amphibians, marsupials and nonmammalian tetrapods, the leucine at residue 8 is substituted for isoleucine. Acting in classical endocrine fashion, Oxytocin elicits regulatory effects by binding specific cell surface receptors which in turn initiate a secondary intracellular response cascade via a phosphoinositide signaling pathway.\u003cbr\u003e\nCross Reactivity\u003cbr\u003e\nCompound\u003cbr\u003e\n% Cross Reactivity\u003cbr\u003e\nMesotocin\u003cbr\u003e\nOxytocin\u003cbr\u003e\n-Vasotocin\u003cbr\u003e\n-Oxytocin\u003cbr\u003e\nSomatostatin\u003cbr\u003e\nMet-Enkephalin\u003cbr\u003e\n-Vasopressin\u003cbr\u003e\n-Vasopressin\u003cbr\u003e\nGrowth Hormone\u003cbr\u003e\nTocinoic acid\u003cbr\u003e\nMelanostatin\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nOxytocin also known as the \"love hormone\" is a peptide consisting of nine amino acids with a molecular mass of approximately 1007 Daltons. It is synthesized in the hypothalamus and secreted by the posterior pituitary gland. Additionally oxytocin is expressed in other tissues including the uterus ovaries and even the heart. Its mechanical function involves binding to its receptor the oxytocin receptor (OXTR) which is a G-protein-coupled receptor and triggering intracellular signaling cascades that lead to calcium mobilization muscle contraction and other cellular responses.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nOxytocin plays several significant roles in the body. It is involved in reproductive and social behaviors such as childbirth and lactation. During childbirth oxytocin induces uterine contractions facilitating the labor process. It also promotes milk ejection during breastfeeding by mediating the contraction of myoepithelial cells surrounding the mammary glands. Furthermore oxytocin contributes to social bonding and modulates emotional responses. It acts both in the central nervous system and peripherally affecting various physiological processes.\u003cbr\u003e\nPathways\u003cbr\u003e\nOxytocin is an important player in the neuroendocrine system especially in the pathways regulating labor and lactation. It functions closely with other neuropeptides and hormones such as vasopressin which also shares sequence homology and structural similarities. Oxytocin influences the MAPK\/ERK signaling pathway which impacts cellular growth and differentiation. These pathways highlight oxytocin's role not only in reproductive events but also in the broader context of cell signaling networks within the body.\u003cbr\u003e\nOxytocin has links to conditions like autism spectrum disorders (ASD) and postpartum depression. Researchers suggest that alterations in oxytocin levels or its receptor function may contribute to social and emotional dysfunctions found in ASD. In postpartum depression abnormal oxytocin activities are thought to affect mood and emotional bonding between mother and child. Oxytocin interacts with other proteins like the serotonin receptor influencing mood regulation and emotional stability. Understanding oxytocin’s role in these disorders may lead to potential therapeutic strategies.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843567210665,"sku":"ab133050","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab133050","provider":"Iright","version":"1.0","type":"link"}