{"product_id":"abcam-ab133408","title":"Abcam, ab133408, ECL Substrate Kit (Very High Sensitivity)","description":"\u003cp\u003eSize: 20mL \/ 100mL \/ 200mL\u003cbr\u003e\nVery High Sensitivity ECL Substrate Kit () ab133408 uses a new horseradish peroxidase (HRP) substrate specially developed to take advantage of the large linear dynamic range provided by CCD imaging.\u003cbr\u003e\nProduct details:\u003cbr\u003e\nVery High Sensitivity ECL Substrate Kit () ab133408 uses a new horseradish peroxidase (HRP) substrate specially developed to take advantage of the large linear dynamic range provided by CCD imaging. It is designed for the detection of proteins from 4.6pg to 4.7ng of protein per band.\u003cbr\u003e\nECL Substrate Kit ab133408 produces a strong, extremely long-lived signal, which, combined with very low background levels, allows for long exposure times enabling the detection of low-abundance proteins. Additionally, the signal from the ECL substrate is linear with respect to protein amount over a broad range of concentrations, displaying no substrate depletion at high protein loads, allowing the user to take full advantage of the linear range of the CCD detection method.\u003cbr\u003e\nThe ECL substrate is also compatible with X-ray film detection, though the limited dynamic range of film will make resulting data less quantitative.\u003cbr\u003e\nOur ECL kits include our popular ECL Substrate Kit\u003cbr\u003e\nab65623\u003cbr\u003e\nand our high sensitivity ECL substrate kits:\u003cbr\u003e\n- High Sensitivity ECL Substrate Kit\u003cbr\u003e\nab133406\u003cbr\u003e\nto detect 23pg-187ng of protein per band\u003cbr\u003e\n- this kit (Very High Sensitivity ECL Substrate Kit ab133408) to detect 4.6pg-4.7ng of protein per band\u003cbr\u003e\n- Ultra High Sensitivity ECL Substrate Kit\u003cbr\u003e\nab133409\u003cbr\u003e\nto detect 1.2pg-2ng of protein per band\u003cbr\u003e\nDetection ranges in pg and ng stated above should be used for guidance only as detection range is dependent on the molecular weight of a protein.\u003cbr\u003e\nThis product was previously called Optiblot ECL Max Detect Kit (4.6pg-4.7ng).\u003cbr\u003e\n- 100ml kit for 1000cm\u003cbr\u003e\nmembrane\u003cbr\u003e\n- 200ml kit for 2000cm\u003cbr\u003e\nmembrane\u003cbr\u003e\nThe primary antibody can often be diluted 5 to 10 fold more than usual when using this ECL substrate. A typical primary antibody dilution range using the substrate is 1\/5000 - 1\/20,000, with a typical secondary antibody dilution range of 1\/20,000 - 1\/100,000. Some optimisation may be required.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843650736297,"sku":"ab133408","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab133408","provider":"Iright","version":"1.0","type":"link"}