{"product_id":"abcam-ab134161","title":"Abcam, ab134161, Anti-CD276 antibody [EPNCIR122]","description":"\u003cp\u003eSize: 20µL \/ 100µL \/ 1mL\u003cbr\u003e\nAnti-CD276 antibody [EPNCIR122] (ab134161) is a rabbit monoclonal antibody detecting CD276 in  Western Blot, Flow Cytometry, IP, IHC-Fr, ICC\/IF . Suitable for  Human, Mouse . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 20 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPNCIR122,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Mouse, Human,\u003cbr\u003e\nApplications:ICC\/IF, IHC-Fr, Flow Cyt, WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAnti-CD276 antibody [EPNCIR122] (ab134161) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt, ICC\/IF, IHC-Fr, IP, WB in human, mouse samples.\u003cbr\u003e\nAnti-CD276 antibody [EPNCIR122] (ab134161) has been cited over 20 times in peer reviewed journals and is trusted by the scientific community.\u003cbr\u003e\nAbcam's high quality manufacturing and validation processes ensure Anti-CD276 antibody [EPNCIR122] (ab134161) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.\u003cbr\u003e\nThe specificity of Anti-CD276 antibody [EPNCIR122] (ab134161) has been confirmed by testing in knockout samples.\u003cbr\u003e\nAnti-CD276 antibody [EPNCIR122] (ab134161) specifically detects CD276 (UniProt ID: Q5ZPR3; Molecular weight: 54kDa) and is sold in 100 µL and 1 mL selling sizes.\u003cbr\u003e\nConjugation-ready, carrier free format available for antibody clone EPNCIR122 -\u003cbr\u003e\nab256585\u003cbr\u003e\nAntibody clone EPNCIR122 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488, Alexa Fluor® 594, Alexa Fluor® 555 (\u003cbr\u003e\nab218280\u003cbr\u003e\nab218282\u003cbr\u003e\nab218283\u003cbr\u003e\nThis antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Brad St. Croix.\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nSpecies reactivity\u003cbr\u003e\nRat: We have preliminary internal testing data to indicate this antibody may not react with this species.\u003cbr\u003e\nPlease\u003cbr\u003e\ncontact us\u003cbr\u003e\nfor more information.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nCD276 also known as B7-H3 is an immunoregulatory protein with a mass of approximately 57 kDa although post-translational modifications can increase its observed size. It is a member of the B7 family which is part of the immunoglobulin superfamily. CD276 is widely expressed in various tissues including placenta liver heart and also in many tumor cells. It is observed in lesser amounts in normal tissues making it an interesting target for cancer research. Various assays like HEK293 and HEK293T cell confluency studies often use CD276 to explore its expression under different conditions and seeding densities which are represented in confluency charts.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nCD276 modulates immune responses by acting as a co-inhibitory ligand impacting T-cell proliferation and cytokine synthesis. Not part of a known complex it plays a role in the adaptive immune system by providing negative feedback regulation that can inhibit the immune response. This capability has implications in preventing autoimmunity but may also contribute to tumor immune evasion by reducing effective anti-tumor responses. The B7-H3\/CD276 interaction with receptors such as IL receptor family members suggests its diverse immunomodulatory functions.\u003cbr\u003e\nPathways\u003cbr\u003e\nCD276 participates in the T-cell receptor (TCR) signaling pathways influencing immune checkpoint pathways. It interacts with other immune checkpoint proteins like PD-L1 and CTLA-4 but its exact receptor partner remains unidentified. The presence of CD276 in these pathways highlights its role in controlling T-cell activation and tolerance. By modulating TCR signals and impacting downstream effects CD276 serves as a regulatory point that can balance immune activation and inhibition critical in immune-mediated conditions.\u003cbr\u003e\nCD276 has strong associations with cancer particularly in solid tumors such as prostate and breast cancer. It is often overexpressed in malignant tissues contributing to immune evasion by tumors. Researchers propose it as a potential target for cancer immunotherapy due to this attribute. CD276's link to disorders like asthma has also been suggested where its interaction with proteins involved in inflammatory pathways such as IL response elements can exacerbate the condition. This implicates it as a dual-role protein in both disease progression and potential therapeutic targeting.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855732396201,"sku":"ab134161","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab134161","provider":"Iright","version":"1.0","type":"link"}