{"product_id":"abcam-ab136716","title":"Abcam, ab136716, Rabbit Anti-Rat IgG H\u0026L (beta-galactosidase)","description":"\u003cp\u003eSize: 500µL\u003cbr\u003e\nSuitable for WB, ICC, IHC-Fr, IHC-P, ELISA. Cited in 2 publications.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nTarget species:Rat,\u003cbr\u003e\nTarget isotype:IgG,\u003cbr\u003e\nTarget specificity:Heavy \u0026amp; Light chains,\u003cbr\u003e\nPre-adsorbed:No,\u003cbr\u003e\nConjugation:Beta-galactosidase,\u003cbr\u003e\nApplications:WB, ICC, IHC-Fr, IHC-P, ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nClonality:Polyclonal,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nSpecificity:Beta-galactosidase labelling is well adapted to ELISA and produces no or very little background. It is more difficult to use in immunochemistry where its use can be justified only when the endogenous activities of other available labels produce significant problems.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nPreparation of Substrate Solutions: 0.1M phosphate buffer pH7, 1mM MgSO\u003cbr\u003e\n, 0.2mM MnSO\u003cbr\u003e\n, 2mM Mg titriplex. Add extemporaneously 7µl\/ml beta-mercaptoethanol and 0.9mg\/ml ortho-nitrophenyl-beta-galactopyranoside. Incubate for 30 minutes at 37°C. Stop the reaction with V\/2 volume of 1M Na\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab136716 is purified by a very stringent purification protocol where non-specific and specific purifications alternate (affinity chromatography)., Storage buffer-Constituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847544557737,"sku":"ab136716","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab136716","provider":"Iright","version":"1.0","type":"link"}