{"product_id":"abcam-ab138345","title":"Abcam, ab138345, Recombinant EBV Nuclear Antigen\/EBNA1 protein","description":"\u003cp\u003eSize: 250µg\u003cbr\u003e\nRecombinant EBV Nuclear Antigen\/EBNA1 protein is a Epstein-Barr Virus (Strain B95-8) Full Length protein, in the 1 to 640 aa range with \u0026gt;95% purity and suitable for western blot, SDS-PAGE and ELISA.The predicted molecular weight of ab138345 protein is 63 kDa. - Save time and ensure accurate results- use our EBV Nuclear Antigen\/EBNA1 protein as a control\u003cbr\u003e\nKey facts\u003cbr\u003e\nPurity:\u0026gt;95% SDS-PAGE,\u003cbr\u003e\nExpression system:Escherichia coli,\u003cbr\u003e\nTags:His tag N-Terminus,\u003cbr\u003e\nApplications:SDS-PAGE, WB, ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nBiologically active:No,\u003cbr\u003e\nAccession:P03211,\u003cbr\u003e\nAnimal free:No,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nSpecies:Epstein-Barr Virus (Strain B95-8),\u003cbr\u003e\nStorage buffer:Constituents: Phosphate Buffer, 36% Urea, 5.8% Sodium chloride, 0.1% Poly(oxy-1,2-ethanediyl), .alpha.-tridecyl-.omega.-hydroxy-\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nEnsure the validity of your results using our Recombinant EBV Nuclear Antigen\/EBNA1 protein ab138345 as a positive control in western blot and SDS-PAGE. Analyze your EBV EBNA-1 ELISA data using the ab138345 protein to generate and plot a standard curve.\u003cbr\u003e\nCheck out of western blot protocol for more information\u003cbr\u003e\nCheck out our protein gel staining guide for SDS-PAGE\u003cbr\u003e\nCheck out our ELISA protocol for more information\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nEBV Nuclear Antigen 1 (EBNA1) is a protein expressed by the Epstein-Barr Virus (EBV) also known as Human Herpesvirus 4. It has a molecular weight of approximately 80 kDa and shows expression in latently infected cells. EBNA1 acts as a viral episome-maintenance protein that binds to the EBV genome ensuring viral DNA replication and partitioning during cell division. This protein facilitates the persistent infection of EBV in host cells. Occasionally it is also referred to as EBV EBNA or EBNA-1 protein.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nEBNA1 functions as an EBV-specific DNA-binding protein essential for maintaining the viral episome within infected cells. EBNA1 plays a role in gene regulation by interacting with specific DNA sequences called oriP to assist in the replication and maintenance of the viral DNA. It does not form part of a larger protein complex but strongly influences cellular processes through these interactions. EBNA1 ensures the survival and propagation of the virus without immediate cytopathic effects contributing to its long-term persistence.\u003cbr\u003e\nPathways\u003cbr\u003e\nEBNA1 is central to viral latency and replication pathways in host cells. EBNA1 influences both the EBV lytic and latent cycles maintaining the latency by stabilizing the viral episome within the nucleus of host cells and regulating viral gene expression. It interacts with cellular proteins like USP7 a deubiquitinase important for the regulation of EBNA1 stability and function. These pathways highlight EBNA1's role in the balance between viral latency and active replication.\u003cbr\u003e\nEBNA1 is closely associated with several EBV-related cancers such as Burkitt's lymphoma and nasopharyngeal carcinoma. The persistent expression of EBNA1 contributes to oncogenic processes by disrupting normal cellular control mechanisms. EBNA1's interaction with p53 a tumor suppressor protein can interfere with the host's cell-cycle regulation and apoptosis allowing for malignant transformation. These interactions illustrate EBNA1's significant role in the pathogenesis of disorders linked to EBV infection.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843798093993,"sku":"ab138345","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab138345","provider":"Iright","version":"1.0","type":"link"}