{"product_id":"abcam-ab138668","title":"Abcam, ab138668, Anti-PRAK\/MK5 (phospho T182) antibody","description":"\u003cp\u003eSize: 100µg\u003cbr\u003e\nRabbit Polyclonal PRAK\/MK5 phospho T182 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human MAPKAPK5 phospho T182.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Polyclonal,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:Synthetic Peptide within Human MAPKAPK5 phospho T182. The exact immunogen used to generate this antibody is proprietary information.Q8IW41\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab138668 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation., Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nPRAK also known as MK5 is a serine\/threonine protein kinase with a molecular weight of approximately 54 kDa. It is expressed in various tissues like the heart brain liver and skeletal muscle. PRAK belongs to the mitogen-activated protein kinase (MAPK) signaling pathway and gets activated by phosphorylation. Mechanically PRAK interacts with and phosphorylates other proteins which affects their activity and function playing an important role in regulating cellular processes such as stress response and the cell cycle.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nPRAK\/MK5 is a critical component in the regulation of cellular responses to stress stimuli. It is a participant in the activation loop of the MAPK p38 signaling pathway where it serves to influence the phosphorylation state and activity of downstream effectors. PRAK is not typically known to be part of a large complex but it interacts directly with other kinases to amplify or dampen the stress response signal. This allows cells to adapt to varied stress conditions by modulating gene expression apoptosis and other cell fate decisions.\u003cbr\u003e\nPathways\u003cbr\u003e\nPRAK\/MK5 plays essential roles within the p38 MAPK and ERK signaling pathways. It closely associates with p38 MAPK which phosphorylates and activates PRAK enabling it to further disseminate the signal. In these pathways PRAK interplays with proteins such as MAPKAPK-2 and HSP27 facilitating diverse cellular activities like inflammation and differentiation. These signaling networks allow PRAK to serve as a link between external stimuli and internal cellular outcomes.\u003cbr\u003e\nPRAK\/MK5 influences cancer and cardiovascular diseases. Its activity connects closely with tumor suppression wherein it regulates actions involving proteins like p53 and HSP27 impacting cell cycle arrest and apoptosis. In cardiovascular contexts altered PRAK function can relate to heart disease since it regulates stress-induced cardiac responses. Understanding PRAK's interplay with these proteins sheds light on its potential as a therapeutic target for treating diseases related to stress signaling dysfunction.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46848593920169,"sku":"ab138668","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab138668","provider":"Iright","version":"1.0","type":"link"}