{"product_id":"abcam-ab138874","title":"Abcam, ab138874, Hydrogen Peroxide Assay Kit (Cell-based)","description":"\u003cp\u003eSize: 200Test\u003cbr\u003e\nHydrogen Peroxide Assay Kit (Cell-based) (ab138874) uses our AbGreen Indicator to quantify hydrogen peroxide in live cells.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Fluorescent,\u003cbr\u003e\nSample types:Suspension cells, Adherent cells,\u003cbr\u003e\nAssay type:Cell-based,\u003cbr\u003e\nSensitivity:= 3 µM,\u003cbr\u003e\nAssay time:30m,\u003cbr\u003e\nAssay Platform:Fluorescence microscope\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHydrogen Peroxide Assay Kit (Cell-based) (ab138874) uses our AbGreen Indicator to quantify hydrogen peroxide in live cells. The indicator is cell-permeable, and generates green fluorescence when it reacts with hydrogen peroxide.\u003cbr\u003e\nThe assay is in a \"mix and read\" assay format that is compatible with HTS liquid handling instruments and a 96-well or 384-well plate format. It provides a sensitive, one-step fluorometric assay to detect as little as 0.3 nanomoles of H\u003cbr\u003e\nin a 100 μL assay volume (3 μM). Results can be read by either a fluorescence microplate reader at Ex\/Em = 490\/520 nm for H\u003cbr\u003e\ndetection in solution or a fluorescence microscopy for live cell H\u003cbr\u003e\nimaging.\u003cbr\u003e\nHydrogen peroxide assay protocol summary:\u003cbr\u003e\n- add samples and standards to wells\u003cbr\u003e\n- add green indicator solution\u003cbr\u003e\n- analyze with microplate reader for 15-60 min.\u003cbr\u003e\nAlternatively, for live cell assay:\u003cbr\u003e\n- add green indicator solution to cells\u003cbr\u003e\n- incubate for 5-60 min whilst analyzing with microplate reader or fluorescence microscope\u003cbr\u003e\nHydrogen peroxide (H\u003cbr\u003e\n) is a reactive oxygen metabolic by-product that serves as a key regulator for a number of oxidative stress-related states. It is involved in many biological events that are linked to asthma, atherosclerosis, diabetic vasculopathy, osteoporosis, a number of neurodegenerative diseases and Down's syndrome. The measurement of this reactive species is helpful for determining how oxidative stress modulates various intracellular pathways.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843559870633,"sku":"ab138874","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab138874","provider":"Iright","version":"1.0","type":"link"}