{"product_id":"abcam-ab138879","title":"Abcam, ab138879, Universal Kinase Assay Kit (Fluorometric)","description":"\u003cp\u003eSize: 250Test\u003cbr\u003e\nUniversal Kinase Assay Kit (Fluorometric) (ab138879) is based on monitoring ADP formation, which is directly proportional to enzyme phosphotransferase activity and is measured fluorimetrically.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Fluorescent,\u003cbr\u003e\nSample types:Purified protein, Cell Lysate,\u003cbr\u003e\nAssay type:Enzyme activity,\u003cbr\u003e\nSensitivity:\u0026lt; 0.3 µM,\u003cbr\u003e\nRange:1 - 300 µM,\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nUniversal Kinase Assay Kit (Fluorometric) (ab138879) is based on monitoring ADP formation, which is directly proportional to enzyme phosphotransferase activity and is measured fluorimetrically. This enzyme-coupled kit provides a fast, simple, and homogeneous assay to measure kinase activities. It is a non-radioactive and no wash method to detect the amount of ADP produced from enzyme reaction. Its characteristics of high sensitivity (\u0026lt;0.3 uM ADP) and broad ATP tolerance (1-300 uM) make it an ideal kit for determining kinase Michaelis-Menten kinetics and for screening and identifying kinase inhibitors. The assay can be performed in a convenient 96-well or 384-well microtiter plate format and easily adapted to automation without a separation step.\u003cbr\u003e\nOther Notes\u003cbr\u003e\nProtein kinases are the enzymes that transfer a phosphate group from a phosphate donor to an acceptor amino acid in a substrate protein. Kinases are of great interest to researchers involved in drug discovery. Most of the commercial protein kinase assay kits are based on monitoring either the phosphopeptide formation or the ATP depletion. For the kinase assay kits that are based on the detection of phosphopeptides, one has to spend time and efforts to identify an optimized peptide substrate while the ATP depletion method suffers various interferences due to the use of luciferase that are inhibited or activated by various biological compounds.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Storage information--80°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843556692137,"sku":"ab138879","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab138879","provider":"Iright","version":"1.0","type":"link"}