{"product_id":"abcam-ab139416","title":"Abcam, ab139416, Mitochondria Fraction Western Blot Cocktail","description":"\u003cp\u003eSize: 200µL\u003cbr\u003e\nMitochondria Fraction Western Blot Cocktail (ab139416) is part of the reagents, controls \u0026amp; accessories range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.\u003cbr\u003e\nKey facts\u003cbr\u003e\nApplications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nReacts with:Mouse, Rat, Human,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nStorage buffer:Preservative: 0.02% Sodium azideConstituents: 0.61% Tris, 0.5% BSA, 0.015% Dodecyl maltoside\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nMitochondria Fraction Western Blot Cocktail (ab139416) contains 3 Mouse mAbs each targeting a specific organelle marker. The presence of mitochondria is determined by Anti-ATP5A ; cytosol by Anti-GAPDH; and nucleus by Anti-Histone H3 (di methyl K9). This cocktail is suitable for determining the purity of organelle isolates prior to further characterization.\u003cbr\u003e\nThis product is particularly valuable to researchers working in organelle proteomics. Mass spectrometry is frequently used in this field to determine the protein content of targeted organelle isolates. These isolates are obtained using differential centrifugation, density gradient fractionation, biochemical enrichment, or affinity purification. Unfortunately, the various methods of purification available for organelle isolation are imperfect and leave behind contaminants from undesired regions of the cell. These contaminants are inevitable, but being aware of which contaminants are present is crucial for analysis of mass spectrometry results. The high sensitivity and species cross reactivity of the antibodies in this cocktail will quickly and easily reveal impurities caused by imperfect sample preparation.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nATP5A GAPDH and Histone H3 are significant in cellular processes. ATP5A part of the ATP synthase complex plays a role in ATP production in mitochondria located on the inner mitochondrial membrane. It weighs approximately 55 kDa. GAPDH or glyceraldehyde-3-phosphate dehydrogenase functions in glycolysis and acts as a housekeeping protein typically found in the cytoplasm and has a mass of about 37 kDa. Histone H3 essential for DNA packaging in the nucleus weighs around 15 kDa and helps structure chromatin. Together they form a 'mitochondrial cocktail' in various studies involving different mitochondrial markers and fractions often used in western blot analyses to examine the color of mitochondria.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nATP5A GAPDH and Histone H3 are involved in essential cellular functions. ATP5A is a component of the F1F0 ATP synthase complex within mitochondria facilitating ATP generation during oxidative phosphorylation which reflects mitochondrial size relevance. GAPDH plays a role in the glycolytic pathway transforming glucose into ATP and NADH. Histone H3 is important in epigenetic regulation affecting gene expression by changing chromatin configuration. While each protein has a distinct role together they provide insights into mitochondrial function and energy metabolism.\u003cbr\u003e\nPathways\u003cbr\u003e\nATP5A GAPDH and Histone H3 influence metabolic and regulatory processes. ATP5A is integral to the oxidative phosphorylation pathway working in concert with proteins like cytochrome c oxidase and other mitochondrial fractions. GAPDH participates in the glycolysis pathway linking to enzymes such as hexokinase and phosphofructokinase. Histone H3 influences gene expression by interacting with transcription factors and modifying enzymes affecting pathways regulating cell cycle and development. These proteins offer a 'cardio cocktail' approach for studying interconnected metabolic pathways used for mitochondria marker assessments.\u003cbr\u003e\nATP5A GAPDH and Histone H3 relate to various pathological conditions. Dysfunction in ATP5A can result in mitochondrial disorders like mitochondrial myopathy impacting muscle function. GAPDH dysregulation associates with Alzheimer's disease highlighting links with other proteins like tau and amyloid-beta implicated in neurodegenerative processes. Alterations in Histone H3 can drive cancer progression by misregulating genes controlling cell proliferation connected to proteins involved in the cell cycle such as p53. These insights provide a foundation for developing therapeutic strategies that target the underlying molecular mechanisms.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847573852329,"sku":"ab139416","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab139416","provider":"Iright","version":"1.0","type":"link"}