{"product_id":"abcam-ab139465","title":"Abcam, ab139465, PTP1B Inhibitor Screening Assay Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nPTP1B Inhibitor Screening Assay Kit (ab139465) is a colorimetric, non-radioactive assay designed to measure the phosphatase activity of purified PTP1B.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Purified protein, Inhibitor compounds,\u003cbr\u003e\nAssay type:Enzyme activity,\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nPTP1B Inhibitor Screening Assay Kit (ab139465) is a colorimetric, non-radioactive assay designed to measure the phosphatase activity of purified PTP1B. This 96-well assay is useful for screening inhibitors and modulators of PTP1B activity.\u003cbr\u003e\nThe kit includes human, recombinant PTP1B (residues 1-322; MW=37.4 kDa), expressed in\u003cbr\u003e\nE. coli\u003cbr\u003e\nas a positive control. The PTP1B inhibitor suramin is including as control for inhibitor detection.\u003cbr\u003e\nThe detection of free-phosphate released is based on the classic Malachite green assay and offers the advantages of convenient, one-step detection and excellent sensitivity, without radioactivity.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nProtein tyrosine phosphatase 1B (PTP1B) is an enzyme involved in the dephosphorylation of tyrosine residues in proteins. Known also as PTPN1 this protein has a molecular weight of approximately 50 kDa. PTP1B is widely expressed in tissues but is highly present in the liver muscle and adipose tissue. It plays a critical role in regulating signal transduction pathways by removing phosphate groups from phosphotyrosine residues on target proteins. This action generally serves as a 'turn-off' switch for various signaling pathways.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nPTP1B plays important roles in glucose and lipid metabolism. It is not generally considered a part of a complex functioning instead as a singular enzyme. Its main action involves the negative regulation of the insulin signaling pathway by dephosphorylating the insulin receptor as well as its associated substrates. This regulation helps maintain normal levels of insulin sensitivity influencing cellular glucose uptake and metabolism.\u003cbr\u003e\nPathways\u003cbr\u003e\nPTP1B has important functions in the insulin and leptin signaling pathways. It has a regulatory influence in these pathways affecting key metabolic processes. In the insulin signaling pathway PTP1B closely interacts with the insulin receptor as well as with insulin receptor substrates (IRS). Its dephosphorylating activity can lead to decreased insulin sensitivity. In the leptin pathway PTP1B modulates leptin signaling by dephosphorylating the Janus kinase 2 (JAK2) another protein involved in the control of energy balance and metabolism.\u003cbr\u003e\nExcessive activity of PTP1B links with disorders such as type 2 diabetes and obesity. Overactivity of PTP1B can lead to insulin resistance contributing to the pathogenesis of type 2 diabetes. In obesity PTP1B may interfere with the normal activity of leptin a hormone critical for regulating energy balance. Its interaction with the insulin receptor and JAK2 makes PTP1B a target for therapeutic interventions aimed at improving insulin sensitivity and energy homeostasis.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843648868521,"sku":"ab139465","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab139465","provider":"Iright","version":"1.0","type":"link"}