{"product_id":"abcam-ab139476","title":"Abcam, ab139476, ROS\/Superoxide Detection Assay Kit (Cell-based)","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nROS\/Superoxide Detection Assay Kit ab139476 is designed to directly monitor real time reactive oxygen species (ROS) production in live cells using fluorescence microscopy, flow cytometry, or microplate reader. - Compatible with phenol red, FBS and BSA - Kit contains ROS inducer and ROS inhibitor - Cited in over 85 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nSample types:Suspension cells, Adherent cells,\u003cbr\u003e\nAssay type:Cell-based,\u003cbr\u003e\nAssay time:90m,\u003cbr\u003e\nAssay Platform:Flow cytometer, Fluorescence microscope\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nROS\/Superoxide Detection Assay Kit ab139476 is designed to directly monitor real time reactive oxygen species (ROS) production in live cells using fluorescence microscopy, flow cytometry, or microplate reader.\u003cbr\u003e\nThe ROS\/superoxide assay protocol is based on two fluorescent dyes: Oxidative Stress Detection Reagent (Green, Ex\/Em 490\/525 nm) for the detection of total ROS, and Superoxide Detection Reagent (Orange, Ex\/Em 550\/620 nm).\u003cbr\u003e\nThrough the combination of these two specific fluorescent probes, the kit provides a simple and specific assay for the real-time measurement of global levels of total reactive oxygen species (ROS), and of superoxides, in living cells. The kit is compatible with the major components of tissue culture media (phenol red, FBS and BSA) when used for flow cytometry or fluorescence microscopy analysis.\u003cbr\u003e\nROS\/superoxide assay protocol summary:\u003cbr\u003e\n- add ROS inhibitor to negative control cells and incubate for 30 min\u003cbr\u003e\n- add ROS\/superoxide detection mix (with the addition of ROS inducer for positive control cells) and incubate for 30-60 min\u003cbr\u003e\n- remove and discard the detection mix liquid\u003cbr\u003e\n- wash samples and analyze with fluorescence microscope, or analyze by flow cytometry or microplate reader without washing\u003cbr\u003e\nReagents provided in the kit are sufficient for at least 200 tests using Fluorescent Microscopy or 50 tests using Flow Cytometry. **Related products** Review the , or the full to learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader. To measure reactive oxygen species within cells, we recommend . Alternative ROS assays are available in orange (\u003cbr\u003e\nab186028\u003cbr\u003e\n), red (\u003cbr\u003e\nab186027\u003cbr\u003e\n), and deep red (\u003cbr\u003e\nab186029\u003cbr\u003e\nab238535\u003cbr\u003e\nis used to measure ROS in biofluids, culture supernatants and cell lysates. For assays designed to differentiate ROS, superoxides, and reactive nitrogen species: to assay ROS and superoxides use ab139476; to assay ROS, superoxides, and reactive nitrogen species use\u003cbr\u003e\nab139473\u003cbr\u003e\n; to assay superoxides use\u003cbr\u003e\nab219943\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Storage information--80°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843596996777,"sku":"ab139476","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab139476","provider":"Iright","version":"1.0","type":"link"}