{"product_id":"abcam-ab14083","title":"Abcam, ab14083, Propidium Iodide","description":"\u003cp\u003eSize: 1mL\u003cbr\u003e\nPropidium Iodide (PI) ab14083 is provided in a convenient solution for staining double-stranded DNA. Propidium Iodide (PI) ab14083 is a fluorescent DNA stain that specifically binds to double-stranded DNA where the plasma membrane has been compromised\/ permeabilized. It does not penetrate live cells. - Use in fluorescence microscopy and flow cytometry - Cited in over 50 publications.\u003cbr\u003e\nKey facts\u003cbr\u003e\nApplications:Flow Cyt, Fluorescence MicroscopySee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nStorage buffer:pH: 7.4Constituents: 99.9% PBS, 0.1% Propidium Iodide\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nPropidium Iodide (PI) binds to double-stranded DNA. PI cannot cross intact plasma membrane and therefore will only be present in DNA of cells where the plasma membrane has been compromised\/ permeabilized.\u003cbr\u003e\nFor Microscopy analysis\u003cbr\u003e\n: PI can be viewed using rhodamine(red) filter (λ= 536\/617). Cells will only be stained if the membrane has been permeated, either naturally (non-viable cells) or with detergents (for fluorescent staining).\u003cbr\u003e\nFor Flow Cytometry analysis:\u003cbr\u003e\nPI staining can be monitored in FL2 channel.\u003cbr\u003e\nIf used together as control for Annexin V assays\u003cbr\u003e\nab14082\u003cbr\u003e\n, ab14083 or\u003cbr\u003e\nab14152\u003cbr\u003e\n, PI should be diluted to 250 μg\/ml solution (in PBS) prior use and added as 1 μl\/ Annexin V Assay (0.25μg\/assay).\u003cbr\u003e\nVisit our\u003cbr\u003e\nFAQs page\u003cbr\u003e\nfor tips and troubleshooting.\u003cbr\u003e\nThis product is manufactured by BioVision, an Abcam company and was previously called 1056 Propidium Iodide. 1056-1 is the same size as the 1 ml size of ab14083.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847572279465,"sku":"ab14083","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab14083","provider":"Iright","version":"1.0","type":"link"}