{"product_id":"abcam-ab146379","title":"Abcam, ab146379, Bluo-Gal, Chromogenic beta-galactosidase substrate","description":"\u003cp\u003eSize: 250mg\u003cbr\u003e\nMW 374.2 g\/mol, Purity \u0026gt;98%. Chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Designed as an alternative to X-Gal (ab144388) in blue-white selection of recombinant bacterial colonies with the lac+ phenotype, producing a darker blue color.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCAS number:97753-82-7,\u003cbr\u003e\nPurity:\u0026gt;98%,\u003cbr\u003e\nMolecular weight:374.2 g\/mol,\u003cbr\u003e\nMolecular formula:C14H16BrNO6,\u003cbr\u003e\nNature:Synthetic,\u003cbr\u003e\nSolubility:Soluble in DMSO to 100 mMSoluble in DMF to 100 mM,\u003cbr\u003e\nBiological description:Chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Designed as an alternative to X-Gal (ab144388) in blue-white selection of recombinant bacterial colonies with the lac+ phenotype, producing a darker blue color.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nInstructions for use:\u003cbr\u003e\nAdd 5-Bromo-3-indoxyl- beta-D-galacto-pyranoside (BB1182) as concentrated stock solution (solvent DMSO or DMF) to autoclaved media\u003cbr\u003e\nRecommended working concentration 0.1-0.3 mM (1-3 mL of 100 mM stock solution per L)\u003cbr\u003e\nFor best results also add 0.1-1 mM of the inducer isopropyl-beta-D-thiogalactopyrano-side (IPTG) to growth media\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information-Store in the dark, Store under desiccating conditions, This product is air and light sensitive and impurities can occur as a result of air oxidation or due to metabolism by microbes\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847451332777,"sku":"ab146379","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab146379","provider":"Iright","version":"1.0","type":"link"}