{"product_id":"abcam-ab14714","title":"Abcam, ab14714, Anti-SDHB antibody [21A11AE7]","description":"\u003cp\u003eSize: 100µg\u003cbr\u003e\nAnti-SDHB antibody [21A11AE7] (ab14714) is a mouse monoclonal antibody detecting succinate dehydrogenase complex iron sulfur subunit B in  Western Blot, Flow Cytometry, IHC-Fr, ICC\/IF . Suitable for  Cow, Human, Mouse, Rat . - Clone 21A11AE7 is the most cited clone to SDHB - KO validated for confirmed specificity - Over 270 publications - Trusted since 2004\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Mouse,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:21A11AE7,\u003cbr\u003e\nIsotype:IgG2a,\u003cbr\u003e\nLight chain type:kappa,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Mouse, Rat, Cow, Human,\u003cbr\u003e\nApplications:ICC\/IF, IHC-Fr, Flow Cyt, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nProduct Specifications\u003cbr\u003e\nAnti-SDHB antibody [21A11AE7] (ab14714) is a mouse monoclonal antibody and is validated for use in Flow Cyt, ICC\/IF, IHC-Fr, WB in cow, human, mouse, rat samples.\u003cbr\u003e\nAnti-SDHB antibody [21A11AE7] (ab14714) specifically detects SDHB (UniProt ID: P21912; Molecular weight: 29kDa) and is sold in 100 µg selling sizes.\u003cbr\u003e\nQuality and Validation\u003cbr\u003e\nAbcam's high quality validation processes ensure Anti-SDHB antibody [21A11AE7] (ab14714) has high sensitivity and specificity.\u003cbr\u003e\nThe specificity of Anti-SDHB antibody [21A11AE7] (ab14714) has been confirmed by testing in knockout samples.\u003cbr\u003e\nAnti-SDHB antibody [21A11AE7] (ab14714) has been cited over 276 times in peer reviewed journals and is trusted by the scientific community.\u003cbr\u003e\nAnti-SDHB antibody [21A11AE7] (ab14714) has 12 independent reviews from customers.\u003cbr\u003e\nRelated Products\u003cbr\u003e\nAntibody clone 21A11AE7 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 647, Alexa Fluor® 488, HRP (\u003cbr\u003e\nab197722\u003cbr\u003e\nab197902\u003cbr\u003e\nab197903\u003cbr\u003e\nWant a custom formulation?\u003cbr\u003e\nThis antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purity-IgG fraction, Purification notes-Near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation., Storage buffer-pH: 7.5Preservative: 0.02% Sodium azideConstituents: HEPES buffered saline, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe protein SDHB also known as Succinate Dehydrogenase Complex Iron Sulfur Subunit B plays a mechanical role as a component of the succinate dehydrogenase (SDH) enzyme. This subunit has a molecular mass of approximately 30 kDa. SDHB is part of the mitochondrial inner membrane where it is expressed in high levels across various tissues including heart kidney and muscle. It contributes to the electron transport chain by facilitating the oxidation of succinate to fumarate an important step in energy production through the tricarboxylic acid (TCA) cycle.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nSDHB participates in the succinate dehydrogenase complex acting within both the TCA cycle and the electron transport chain. By transferring electrons to coenzyme Q it helps generate an electrochemical gradient important for ATP synthesis. This complex also called Complex II includes other subunits such as SDHA SDHC and SDHD. Their interactions ensure proper function of metabolic processes within mitochondria bridging the gap between foundational energy metabolism and complex cellular processes.\u003cbr\u003e\nPathways\u003cbr\u003e\nSDHB plays an important role in cellular respiration and metabolic cycles. It resides in the TCA cycle and the mitochondrial electron transport chain connecting its function to energy generation pathways. Specifically SDHB relates closely to SDHA and coenzyme Q all working together to facilitate electron transfer and effective mitochondrial energy output. The transfer of electrons through this pathway highlights its essential contribution to maintaining cellular energy homeostasis.\u003cbr\u003e\nSDHB mutations are associated with conditions such as paraganglioma and pheochromocytoma. These genetic alterations disrupt normal complex II function leading to the accumulation of succinate and succinate-related oncogenesis. In addition to causing tumor development defective SDHB is linked with familial paraganglioma syndromes. Its interaction with proteins such as SDHA and SDHD critical for the succinate dehydrogenase functionality highlights its significant role in maintaining metabolic balance and when defective contributing to disease pathogenesis.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46844153331881,"sku":"ab14714","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab14714","provider":"Iright","version":"1.0","type":"link"}