{"product_id":"abcam-ab150681","title":"Abcam, ab150681, Picro Sirius Red Stain Kit (Connective Tissue Stain)","description":"\u003cp\u003eSize: 30mL \/ 250mL\u003cbr\u003e\nPicro-Sirius Red Stain Kit (Connective Tissue Stain) ab150681 is an easy-to-use histological kit to visualize collagen I and collagen III fibers in addition to muscle in tissue sections. - Specific stain to study collagen networks in different tissues - Suitable for light microscopy and polarized light microscopy - Cited in over 320 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nSample types:Tissue sections,\u003cbr\u003e\nAssay time:1h\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nPicro sirius red staining has traditionally sometimes been used with polarized light to more easily differentiate the collagen fibres from the background. Studies have used the differences between the appearance of different collagen fibres under polarized light to differentiate between different types of collagen fibres; it is unclear if the differences in appearance of the fibres is due to orientation, thickness and packing of the fibres rather than reflecting different types of collagen fibers.\u003cbr\u003e\nPicro sirius red staining protocol summary\u003cbr\u003e\n- Deparaffinize sections if necessary and hydrate in distilled water\u003cbr\u003e\n- Cover sections in picro-sirius red solution and incubate for 60 min\u003cbr\u003e\n- Wash slide with acetic acid solution\u003cbr\u003e\n- Wash slide with absolute alcohol\u003cbr\u003e\n- Dehydrate, clear and mount slide\u003cbr\u003e\nStaining Interpretation\u003cbr\u003e\nLight Microscopy\u003cbr\u003e\nCollagen\u003cbr\u003e\nMuscle Fibers\u003cbr\u003e\nYellow\u003cbr\u003e\nCytoplasm\u003cbr\u003e\nYellow\u003cbr\u003e\nPolarized Light Microscopy\u003cbr\u003e\nType I (Thick fibers)\u003cbr\u003e\nYellow-Orange Birefringence\u003cbr\u003e\nType III (Thin fibers)\u003cbr\u003e\nGreen Birefringence\u003cbr\u003e\nControl Tissue:\u003cbr\u003e\nLung, Uterus, Muscle, Kidney.\u003cbr\u003e\nGetting the best performance from ab150681\u003cbr\u003e\nab150681 may not be suitable for frozen sections.\u003cbr\u003e\nRelated and recommended products\u003cbr\u003e\nSee below other connective tissue stains to highlight and identify different types of connective tissue in tissue samples with different dyes:\u003cbr\u003e\n- Trichrome Stain Kit (Connective Tissue Stain)\u003cbr\u003e\nab150686\u003cbr\u003e\n- Phosphotungstic Acid Hematoxylin (PTAH) Stain Kit (Connective Tissue Stain)\u003cbr\u003e\nab150683\u003cbr\u003e\n- Reticulum Stain Kit (Connective Tissue Stain)\u003cbr\u003e\nab150684\u003cbr\u003e\nOther Notes\u003cbr\u003e\nPlease note, the 250 mL selling size, was previously described as 100 Test. There has been no change to the physical product.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Ambient - Cannot Ship with Ice, Appropriate short-term storage conditions-Ambient, Appropriate long-term storage conditions-Ambient, Storage information-Ambient\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nCollagen I and III are important structural proteins within the extracellular matrix providing tensile strength and structural support to tissues. Collagen I often called Type I collagen has a molecular mass of approximately 300 kDa. It is expressed abundantly in skin bone tendons and other connective tissues. Collagen III known as Type III collagen is generally found alongside Type I particularly in skin blood vessels and internal organs. Both collagens are essential for maintaining tissue integrity with muscle fibers interacting closely to support proper muscle function.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nCollagen fibers are significant for tissue architecture and are involved in wound healing fibrosis and tissue regeneration. They are components of a complex network within the extracellular matrix that involves proteoglycans and other matrix proteins. Collagens also serve as ligands for cellular receptors like integrins which influence cell adhesion and signaling processes. Picrosirius red is a commonly used stain for identifying collagen fibers in histological studies as it enhances the visibility of these fibers under polarized microscopy.\u003cbr\u003e\nPathways\u003cbr\u003e\nThese collagens play roles in both the Wnt signaling pathway and the transforming growth factor-beta (TGF-β) pathway. They are important for tissue development and repair directly interacting with factors like fibronectin and matrix metalloproteinases. The interactions within these pathways regulate not only synthesis and degradation of the extracellular matrix but also influence cellular behaviors such as migration proliferation and differentiation.\u003cbr\u003e\nCollagen I and III are associated with fibrotic diseases and connective tissue disorders. Mutations or dysregulations in collagen production can lead to conditions like Ehlers-Danlos syndrome characterized by connective tissue fragility or pulmonary fibrosis which involves excessive deposition of extracellular matrix components. Aberrations in these collagens can also disrupt interactions with proteins like elastin impacting tissue elasticity and leading to further pathological conditions.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843537129641,"sku":"ab150681","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab150681","provider":"Iright","version":"1.0","type":"link"}