{"product_id":"abcam-ab151823","title":"Abcam, ab151823, Recombinant Human Cytochrome P450 Reductase protein","description":"\u003cp\u003eSize: 20µg \/ 50µg\u003cbr\u003e\nRecombinant Human Cytochrome P450 Reductase protein is a Human Full Length protein, in the 1 to 679 aa range, expressed in Baculovirus infected Sf9 cells, with \u0026gt;95%, suitable for SDS-PAGE, WB.\u003cbr\u003e\nKey facts\u003cbr\u003e\nPurity:\u0026gt;95% SDS-PAGE,\u003cbr\u003e\nExpression system:Baculovirus infected Sf9 cells,\u003cbr\u003e\nTags:GST tag N-Terminus,\u003cbr\u003e\nApplications:WB, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nBiologically active:No,\u003cbr\u003e\nAccession:P16435,\u003cbr\u003e\nAnimal free:No,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nSpecies:Human,\u003cbr\u003e\nStorage buffer:pH: 7.5Constituents: 25% Glycerol (glycerin, glycerine), 0.79% Tris HCl, 0.31% Glutathione, 0.29% Sodium chloride, 0.04% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nCytochrome P450 reductase also known as NADPH--cytochrome P450 reductase (CPR) is an enzyme important in electron transfer. It has a mass of approximately 77 kDa and is responsible for transferring electrons from NADPH to cytochrome P450 enzymes supporting their function. The enzyme is predominantly expressed in the endoplasmic reticulum of liver cells and other tissues where detoxification processes occur. Cytochrome reductase as it is sometimes called plays a fundamental role in the metabolism of drugs and the synthesis of cholesterol steroids and other lipids.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nCPR facilitates electron donation to all microsomal cytochrome P450 enzymes which are involved in a variety of oxidative reactions. This protein is essential for P450 activity assay as it is part of a multienzyme complex that metabolizes exogenous and endogenous compounds efficiently. Without cytochrome P450 reductase the catalytic activity of cytochrome P450 enzymes becomes impaired affecting metabolizing mechanisms for various substances and having systemic biological impacts.\u003cbr\u003e\nPathways\u003cbr\u003e\nCytochrome P450 reductase directly participates in drug metabolism and steroid biosynthesis pathways. It together with the cytochrome P450 enzymes metabolizes drugs to make them more water-soluble for excretion. The proteins 3 beta-hydroxysteroid dehydrogenase and CYP3A4 are some of the related components within these pathways working in conjunction to achieve metabolic homeostasis. P450 reductase assays are often utilized to study these pathways further understanding the biological roles and interactions of these proteins.\u003cbr\u003e\nCPR has a significant connection to conditions like congenital adrenal hyperplasia and prostate cancer. Mutations or dysregulations in cytochrome P450 reductase can lead to accumulated steroid precursors and disrupted steroid hormone synthesis contributing to these disorders. The relationship with adrenal enzyme 21-hydroxylase and the androgen receptor illustrates the pathway complexities and the clinical relevance of cytochrome P450 reductase. Understanding these connections highlights the importance of CPR in maintaining normal physiological functions and how its abnormalities can lead to serious health issues.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843932836009,"sku":"ab151823","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab151823","provider":"Iright","version":"1.0","type":"link"}