{"product_id":"abcam-ab155446","title":"Abcam, ab155446, Human XIAP ELISA Kit (API3)","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman XIAP ELISA Kit (API3) is a Sandwich (quantitative) ELISA for the measurement of Human XIAP (API3) in Human, Mouse in Biofluids, Cell Culture Media samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Plasma, Cell culture supernatant, Serum,\u003cbr\u003e\nReacts with:Mouse, Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:\u0026lt; 0.25 ng\/mL,\u003cbr\u003e\nRange:2.5 - 600 ng\/mL,\u003cbr\u003e\nAssay Platform:Microplate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAbcam's XIAP Human ELISA kit (Enzyme-Linked Immunosorbent Assay) kit is an\u003cbr\u003e\nin vitro\u003cbr\u003e\nenzyme-linked immunosorbent assay designed for the quantitative measurement of Human XIAP in serum, plasma and cell culture supernatants.\u003cbr\u003e\nThis assay employs an antibody specific for Human XIAP coated on a 96-well plate. Standards and samples are pipetted into the wells and XIAP present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human XIAP antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of XIAP bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.\u003cbr\u003e\nOptimization may be required with urine samples.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nXIAP also known as X-linked inhibitor of apoptosis protein is a member of the inhibitor of apoptosis protein (IAP) family. XIAP has a molecular mass of approximately 57 kDa. This protein inhibits apoptosis by binding to and inhibiting caspases particularly caspase-3 caspase-7 and caspase-9. XIAP expresses in a variety of tissues including the heart kidney and liver but also shows expression in cancer cells. Its role in inhibiting cell death makes it a significant focus for cancer research.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe function of XIAP involves regulation of apoptosis an important process in maintaining cellular homeostasis. It forms part of the apoptosome complex by interacting with caspases to prevent premature cell death. XIAP serves to protect cells from stress-induced apoptotic signals helping in survival under adverse conditions. This ability makes it a subject of interest in studying cancer survival mechanisms since overactive XIAP can allow cancer cells to avoid programmed cell death.\u003cbr\u003e\nPathways\u003cbr\u003e\nApoptosis regulation and cell survival pathways prominently feature XIAP. Particularly XIAP plays a role in the intrinsic apoptotic pathway which is activated in response to internal stress signals. XIAP regulates this pathway via its interaction with caspase-9 and interactions with other proteins such as Smac\/DIABLO which can antagonize XIAP's function. This interaction is key in balancing cell death and survival therefore highlighting its role in apoptosis control.\u003cbr\u003e\nXIAP's dysregulation links to cancer and neurodegenerative disorders. Overexpression of XIAP can contribute to cancer by enabling cells to evade apoptosis leading to uncontrolled cell proliferation. Moreover studies have suggested a role in neurodegenerative diseases where alterations in XIAP may affect neuronal cell survival. In these contexts XIAP's interactions with proteins such as caspases and Smac\/DIABLO surface as important in understanding how apoptosis evasion or enhancement contributes to disease pathology.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855626260649,"sku":"ab155446","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab155446","provider":"Iright","version":"1.0","type":"link"}