{"product_id":"abcam-ab155623","title":"Abcam, ab155623, Recombinant human DKK1 protein","description":"\u003cp\u003eSize: 100µg\u003cbr\u003e\nRecombinant human DKK1 protein is a Human Full Length protein, in the 32 to 266 aa range, with \u0026gt;95% purity, \u0026lt; 1 EU\/µg endotoxin level and suitable for SDS-PAGE and Functional studies. The predicted molecular weight of ab155623 protein is 28.5 kDa. - Save time and ensure accurate results - use our DKK-1 protein as a control - Optimal protein bioactivity, stability and reproducibility\u003cbr\u003e\nKey facts\u003cbr\u003e\nPurity:\u0026gt;95% SDS-PAGE,\u003cbr\u003e\nEndotoxin level:\u0026lt; 1 EU\/µg,\u003cbr\u003e\nExpression system:HEK 293 cells,\u003cbr\u003e\nTags:His tag C-Terminus,\u003cbr\u003e\nApplications:SDS-PAGE, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nBiologically active:Yes,\u003cbr\u003e\nBiological activity:Measured by the ability to inhibit Wnt3-a induced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells. The ED50 for this effect is typically 0.01-0.12 µg\/ml in the presence of 10 ng\/ml of rmWnt3a.,\u003cbr\u003e\nAccession:O94907,\u003cbr\u003e\nAnimal free:No,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nSpecies:Human,\u003cbr\u003e\nReconstitution:Reconstitute with sterile deionized water to a concentration of 200 µg\/ml.,\u003cbr\u003e\nStorage buffer:pH: 7.4Constituents: PBS, 5% Trehalose\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nEnsure the validity of your result using our bioactive recombinant human DKK-1 protein ab155623 as a positive control in SDS-PAGE.\u003cbr\u003e\nThe human DKK-1 protein has a calculated MW of 28.5 kDa. The ab155623 protein migrates as 35 - 45 kDa under reducing condition in SDS-PAGE due to glycosylation.\u003cbr\u003e\nCheck out our protein gel staining guide for SDS-PAGE\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe DKK1 protein also known as Dickkopf-1 is a secreted protein with a mass of approximately 28-30 kDa. It plays a mechanical role in inhibiting the Wnt signaling pathway by binding to LRP5\/6 co-receptors preventing Wnt ligands from activating the pathway. DKK1 is expressed in various tissues including the bone brain and placenta reflecting its involvement in several biological processes. Its capacity to bind and neutralize the Wnt pathway components cements DKK1's role as a significant modulator of cellular functions.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe inhibition of the Wnt signaling by DKK1 alters cell proliferation differentiation and migration. DKK1 does not function as part of a large multi-protein complex but interacts directly with specific co-receptors and modulates the activity of key signaling molecules. This regulatory mechanism controls processes like bone formation and neurogenesis indicating DKK1's broad impact in development and tissue homeostasis. Its expression pattern and biological role position it as an important player in maintaining proper cellular signaling environments.\u003cbr\u003e\nPathways\u003cbr\u003e\nDKK1 is a critical player in the Wnt signaling and bone remodeling pathways. Through its interaction with Wnt pathway components like LRP5\/6 DKK1 modulates these pathways affecting downstream proteins such as β-catenin and influencing gene transcription. In the bone remodeling context DKK1's ability to regulate osteoblast activity connects it to other related proteins like sclerostin contributing to bone density and health.\u003cbr\u003e\nElevated levels of DKK1 are observed in conditions like osteoporosis and cancer. In osteoporosis DKK1's inhibition of Wnt signaling leads to reduced bone formation implicating it alongside proteins like sclerostin in deteriorating bone health. In cancer DKK1's dysregulation can promote tumorigenesis by altering cell signaling and microenvironment interactions. These roles underline the potential of targeting DKK1 for therapeutic purposes in treating these diseases.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843834237097,"sku":"ab155623","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab155623","provider":"Iright","version":"1.0","type":"link"}